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69025 T7•Tag® Affinity Purification Kit

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Catalog NumberPackaging Qty/Pack
69025-3 Glass bottle 1 kit
OverviewThe T7•Tag® Affinity Purification Kit is designed for rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence (e.g., the 11 amino terminal aa of the T7 gene 10 protein). Purification is based on binding target proteins to T7•Tag Monoclonal Antibody that is covalently coupled to cross-linked agarose beads, washing away unbound proteins, and eluting at pH 2.2. Neutralization Buffer is included to limit protein exposure to low pH. Capacity will vary somewhat between different target proteins, but the beads are standardized to bind a minimum of 300 µg T7•Tag β-galactosidase per milliliter of settled resin. The beads can be used in either batch or column methods and can be regenerated a minimum of five times without loss of binding activity.
Catalogue Number69025
Brand Family Novagen®
Product Information
Quality LevelMQ400
Biological Information
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Global Trade Identification Number
Catalog Number GTIN


T7•Tag® Affinity Purification Kit SDS


Safety Data Sheet (SDS) 

T7•Tag® Affinity Purification Kit Certificates of Analysis

TitleLot Number


Protein Purification and Detection Tools


  • Hayakawa, H., et al. 2007. Soluble Journal of Biological Chemistry 282, 26369.
  • Chang-Sik Oh and Steven V. Beer. 2007. Plant Physiology 145, 426.
  • Sukumar, N., et al. 2007. Journal of Bacteriology 189, 3695.
  • Qiu, R., et al. 2005. Journal of Biological Chemistry 280, 16695.
  • Suzuki, T., et al. 2005. Plant and Cell Physiology 46, 1452.
  • Michael H. Kagey, Tiffany A. Melhuish and David Wotton. 2003. The Cell 113, 127.
  • User Protocols

    TB055 pET System Manual
    TB125 T7•Tag® Affinity Purification Kit