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69839 T4 DNA Ligase - Novagen

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Catalog NumberPackaging Qty/Pack
69839-3 Glass bottle 100 u
OverviewT4 DNA Ligase catalyzes the formation of phosphodiester bonds between 3′-hydroxyl and 5′-phosphate groups of adjacent DNA and RNA nucleotides in blunt-end or cohesive-end configurations. The enzyme joins RNA and DNA strands only in duplex molecules and does not join single-stranded nucleic acids. Novagen T4 DNA Ligase is rigorously tested in a blue-white cloning assay under conditions that maximize exposure of DNA ends to any contaminating nucleases. The enzyme is qualified for any ligation or cloning application.
Catalogue Number69839
Brand Family Novagen®
Product Information
Unit of Definition4 Weiss units/µl; one Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of ³²P from pyrophosphate into ATP as Norit®-absorbable material in 20 minutes at 37°C. 0.01 Weiss unit is the amount of enzyme required to ligate > 95% of 1 µg bacteriophage λ <i>Hin</i>d III fragments at 16°C in 20 minutes.
Quality LevelMQ200
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Safety Information according to GHS
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Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
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