Millipore Sigma Vibrant Logo

Steritop™ Filter Units

Steritop bottle top filter units provide a large filter area for rapid sterile filtration of aqueous fluids into narrow-mouth (33 mm) or wide-mouth (45 mm) bottles.

Steritop bottle top filter units provide a large filter area for rapid sterile filtration of aqueous fluids into narrow-mouth (33 mm) or wide-mouth (45 mm) bottles. A small tab inside the funnel secures an optional prefilter in-place during filtration. For added protection against contamination, the threads for bottle attachment have been slightly recessed in case the funnel accidentally touches a work surface.

Overview

Specifications

Ordering Information

Units with fast flow and low protein binding 0.22 µm Millipore Express PLUS (PES) membrane for sterilization of aqueous solutions*Clear Sorting & Filtering Show Filter
Catalog NumberDescriptionFunnel CapacityFitting OutletPack Size
SCGPS01RESteritop Threaded Bottle Top Filter 150 mL 33 mm thread 12 pack, GP 0.22 µm Polyethersulfone 150 mL 33 mm Radio-sterilized Show Pricing & Availability
SCGPS02RESteritop Threaded Bottle Top Filter 250 mL 33 mm thread 12 pack, GP, 0.22 µm, polyethersulfone, 250 mL 33 mm, radio-sterilized Show Pricing & Availability
SCGPS05RESteritop Threaded Bottle Top Filter 500 mL 33 mm thread 12 pack, GP, 0.22 µm, polyethersulfone, 500 mL 33 mm, radio-sterilized Show Pricing & Availability
SCGPT01RESteritop Threaded Bottle Top Filter 150 mL 45 mm thread 12 pack, GP, 0.22 µm, polyethersulfone, 150 mL 45 mm, radio-sterilized Show Pricing & Availability
SCGPT02RESteritop Threaded Bottle Top Filter 250 mL 45 mm thread 12 pack, GP, 0.22 µm, polyethersulfone, 250 mL 45 mm, radio-sterilized Show Pricing & Availability
SCGPT05RESteritop Threaded Bottle Top Filter (this item has been replaced with S2GPT05RE) 500 mL 45 mm thread 12 pack, GP, 0.22 µm, polyethersulfone, 500 mL 45 mm, radio-sterilized Show Pricing & Availability
SCGPT10RESteritop Threaded Bottle Top Filter 1 L 45 mm thread 12 pack, GP, 0.22 µm, polyethersulfone, 1000 mL 45 mm, radio-sterilized Show Pricing & Availability

* These products have been tested and validated for use in stem cell research applications. To determine their effects on Mouse Stem Cell growth or differentiation, three lots of Steritop-GP devices were used to filter media with LIF. Once filtered, this media was used to passage mouse stem cells five times to verify that Steritop-GP filtration does not impact pluripotency of mouse stem cells.

Back To Top

Receiver and storage bottles (to be ordered separately)Clear Sorting & Filtering Show Filter
Catalog NumberDescriptionProcess VolumeFitting InletPack Size
SC00B02REStericup Receiver Flask 250 mL 45 mm thread 12 Show Pricing & Availability
SC00B05REStericup Receiver Flask 500 mL 45 mm thread 12 Show Pricing & Availability
SC00B10REStericup Receiver Flask 1000 mL 45 mm thread 12 pack, Receiver Flask, 1000 mL, radio-sterilized Show Pricing & Availability

Back To Top

Documentation

Data Sheet

Title
Stericup and Steritop Vacuum Filter Cups

FAQ

QuestionAnswer
Do you have a filter for 100% serum?Yes, you can filter serum with Millipore Express membrane. The Express membrane was designed for this application and offers fast filtration time. Millipore Express is available in a large number of filter devices including Millex syringe filters, Stericup and Steritop vacuum devices, Sterivac for large volumes of media and Steriflip for 50 mls.
I want to sterilize 100% ethanol. Are any of the Stericups or Steritops compatible with ethanol?The funnel and receiver flask is made of polystyrene. Any molded polystyrene will craze in pure ethanol and could structurally fail depending on the forces being applied to it. The funnel could handle the short exposure time but the receiver would craze eventually. Therefore we would not recommend the Stericup with 100% ethanol. The Steritop, if you keep the exposure time to the funnel minimal, should be fine. As you dilute the alcohol the compatibility improves.

Both Express and Durapore membranes are compatible. MCE is not.
I will be filtering tissue culture media with your Stericup/top and am concerned that wetting agents in the filter could effect my cells. Which membrane type would you recommend to avoid this?All of Millipore's Tissue Culture Media filtration sterilization devices contain membranes that are wetting agent (surfactant ) free.
When I use my Stericup/top, sometimes some of my sterilized solution gets pulled into my vacuum line. How can I eliminate this from happening?Attach your vacuum tubing and turn the vacuum on before adding the liquid to the Stericup/top. This eliminates any initial solution that flowed through the membrane before starting the vacuum from being pulled into the vacuum line.
What is the diameter of the membrane in the Stericup and Steritop units?All of the cups and tops currently have a 70mm diameter membrane, which has 43 cm2 of surface area.
Should I filter my acrylamide solution prior to gel casting?Yes, filtering your acrylamide solution before adding the polymerization activator ammonium persulfate and TEMED will accomplish two things.
1. It will eliminate particulate matter that may interfere with base calling when sequencing or that may create "hot" spots in the gel, and
2. It will aid in degassing, which will promote efficient polymerization of the gel.

Products appropriate for this application would be Stericup, Steritop or Steriflip with express membrane.
What type of flow rates can I expect with the Stericups and Steritops?For typical non -serum tissue culture media you can expect flow rates from 1-1.5 lpm.
Are Stericup/tops compatible with formamide?Yes. Formamide is probably the least organic compound. It's almost like water. So it would be compatible with everything. The potential mistake is mistaking formamide for dimethylformamide, which is a powerful organic solvent.
Are Stericup and Steritop units autoclavable?No. The highest temperature that the product can be used at is 50C ( 122F ).
Can I bubble point my Stericup/top?No. The unit is not designed to handle the pressures required to perform a bubble point test. It is designed to be run under vacuum only (15 psi max). Bubble points for any of the membranes in the Stericups/tops need to be run at pressures ranging from 22 psi to 57psi.

User Guides

Title
Stericup and Steritop Filtration and Storage Systems

Related Products & Applications

Product Families

Related Products By: Application Facete

Related Products By: Brand Facete

Categories

Life Science Research > Cell Culture and Systems > Sterile Filtration Devices