|Stericup and Steritop Vacuum Filter Cups|
|Do you have a filter for 100% serum?||Yes, you can filter serum with Millipore Express membrane. The Express membrane was designed for this application and offers fast filtration time. Millipore Express is available in a large number of filter devices including Millex syringe filters, Stericup and Steritop vacuum devices, Sterivac for large volumes of media and Steriflip for 50 mls.|
|I want to sterilize 100% ethanol. Are any of the Stericups or Steritops compatible with ethanol?||The funnel and receiver flask is made of polystyrene. Any molded polystyrene will craze in pure ethanol and could structurally fail depending on the forces being applied to it. The funnel could handle the short exposure time but the receiver would craze eventually. Therefore we would not recommend the Stericup with 100% ethanol. The Steritop, if you keep the exposure time to the funnel minimal, should be fine. As you dilute the alcohol the compatibility improves.
Both Express and Durapore membranes are compatible. MCE is not.
|I will be filtering tissue culture media with your Stericup/top and am concerned that wetting agents in the filter could effect my cells. Which membrane type would you recommend to avoid this?||All of Millipore's Tissue Culture Media filtration sterilization devices contain membranes that are wetting agent (surfactant ) free.|
|When I use my Stericup/top, sometimes some of my sterilized solution gets pulled into my vacuum line. How can I eliminate this from happening?||Attach your vacuum tubing and turn the vacuum on before adding the liquid to the Stericup/top. This eliminates any initial solution that flowed through the membrane before starting the vacuum from being pulled into the vacuum line.|
|What is the diameter of the membrane in the Stericup and Steritop units?||All of the cups and tops currently have a 70mm diameter membrane, which has 43 cm2 of surface area.|
|Should I filter my acrylamide solution prior to gel casting?||Yes, filtering your acrylamide solution before adding the polymerization activator ammonium persulfate and TEMED will accomplish two things.
1. It will eliminate particulate matter that may interfere with base calling when sequencing or that may create "hot" spots in the gel, and
2. It will aid in degassing, which will promote efficient polymerization of the gel.
Products appropriate for this application would be Stericup, Steritop or Steriflip with express membrane.
|What type of flow rates can I expect with the Stericups and Steritops?||For typical non -serum tissue culture media you can expect flow rates from 1-1.5 lpm.|
|Are Stericup/tops compatible with formamide?||Yes. Formamide is probably the least organic compound. It's almost like water. So it would be compatible with everything. The potential mistake is mistaking formamide for dimethylformamide, which is a powerful organic solvent.|
|Are Stericup and Steritop units autoclavable?||No. The highest temperature that the product can be used at is 50C ( 122F ).|
|Can I bubble point my Stericup/top?||No. The unit is not designed to handle the pressures required to perform a bubble point test. It is designed to be run under vacuum only (15 psi max). Bubble points for any of the membranes in the Stericups/tops need to be run at pressures ranging from 22 psi to 57psi.|
|Stericup and Steritop Filtration and Storage Systems|