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F8205-BC Sebomed Basal Medium

F8205-BC
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
F8205 500 ml
Description
OverviewModified DMEM/Ham's F-12 (1:1), was optimized to grow the human sebaceous gland cell line SZ95 in vitro. The cell line itself was developed by transfecting primary human facial sebocytes. This defined medium is ideal for controlled studies regarding the effects of hormones, cytokines,xenobiotics, Ca2+ etc. on proliferation, differentiation, lipid synthesis, and cytokine production in the cell culture system. The Ca2+ concentration was kept low (0.05 mM)in order to cultivate sebocytes, and keratinocytes under similar culture conditions with or without addition of Ca2+. The basal medium, which is free of bovine products, does not contain phenol red or linoleic acid (or any other fatty acid).

Specifications:

Sterility: Pass
BHK 21 Cell Proliferation Test: Pass
BHK 21 Cell Cloning Efficicency Test: Pass
pH: 7.1-7.4
Osmolality: 0.275-0.305 Osm/kg H20
Color: Light pink
Appearance: Clear

Recommendations:
For optimal performance, this basal medium needs to be supplemented with 0.1 ng/ml Epidermal Growth Factor (EGF ; cat. no. W 1325950500), and 0.1 ml/ml Fetal Bovine Serum (FBS). Gentamycin sulphate (cat. no. A2710/2) at a concentration of 50 mg/ml should be used to protect against bacterial contamination or 100 U/100 mg/l Penicillin/Streptomycin (cat. no. A2210/2)
Catalogue NumberF8205-BC
Brand Family Calbiochem®
References
Product Information
Quality LevelMQ300
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
F8205 04027269840624

Documentation