F8205-BC Sebomed Basal Medium

F8205-BC
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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      F8205
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          500 ml
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          Description
          OverviewModified DMEM/Ham's F-12 (1:1), was optimized to grow the human sebaceous gland cell line SZ95 in vitro. The cell line itself was developed by transfecting primary human facial sebocytes. This defined medium is ideal for controlled studies regarding the effects of hormones, cytokines,xenobiotics, Ca2+ etc. on proliferation, differentiation, lipid synthesis, and cytokine production in the cell culture system. The Ca2+ concentration was kept low (0.05 mM)in order to cultivate sebocytes, and keratinocytes under similar culture conditions with or without addition of Ca2+. The basal medium, which is free of bovine products, does not contain phenol red or linoleic acid (or any other fatty acid).

          Recommendations:
          For optimal performance, this basal medium needs to be supplemented with 0.1 ng/ml Epidermal Growth Factor (EGF ; cat. no. W 1325950500), and 0.1 ml/ml Fetal Bovine Serum (FBS). Gentamycin sulphate (cat. no. A2710/2) at a concentration of 50 mg/ml should be used to protect against bacterial contamination or 100 U/100 mg/l Penicillin/Streptomycin (cat. no. A2210/2)
          Specifications:

          Sterility: Pass
          BHK 21 Cell Proliferation Test: Pass
          BHK 21 Cell Cloning Efficicency Test: Pass
          pH: 7.1-7.4
          Osmolality: 0.275-0.305 Osm/kg H20
          Color: Light pink
          Appearance: Clear

          Catalogue NumberF8205-BC
          Brand Family Calbiochem®
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          Ship Code Blue Ice Only
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
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