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560000 SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem

560000
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Key Specifications Table

CAS #
3483-12-3

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560000-10ML
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      560000-1SET
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          Description
          OverviewConcentrate of Dithiothreitol (DTT; Cat. No. 233155) in phosphate buffer, pH 6.5-7.5. Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum. Each vial is sufficient to make 100 ml of working solution.
          Note: 1 set = 20 x 10 ml.
          Catalogue Number560000
          Brand Family Calbiochem®
          References
          ReferencesHirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
          Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
          Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
          Cleland, W.W. 1964. Biochemistry 3, 480.
          May, J.R. 1953 (September). Lancet p. 535.
          Product Information
          CAS number3483-12-3
          FormLiquid
          Formulation20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSEK1610000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful
          Storage +15°C to +30°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsOnce a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          SPUTOLYSIN® Reagent - CAS 578517 - Calbiochem Certificates of Analysis

          TitleLot Number
          560000

          References

          Reference overview
          Hirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
          Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
          Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
          Cleland, W.W. 1964. Biochemistry 3, 480.
          May, J.R. 1953 (September). Lancet p. 535.

          Citations

          Title
        • I.S. Woolhouse, D.L. Bayley and R.A. Stockley. (2002) Effect of Sputum Processing with Dithiothreitol on the Detectino of Inflammatory Mediators in Chronic Bronchitis and Bronchiectasis. Thorax 57, 667-671.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision28-April-2008 RFH
          DescriptionA concentrate of dithiothreitol (DTT) in phosphate buffer. Useful for the isolation of pathogenic or saprophytic bacteria, fungi, yeast, and epithelial cells from sputum and other mucoid fluids.
          FormLiquid
          Formulation20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.
          Recommended reaction conditionsA. To prepare sputum samples: 1. In a screw-cap centrifuge tube, overlay the sputum sample with an equal volume of the diluted SPUTOLYSIN® Reagent. 2. Suspend the sputum by mixing on a vortex mixer for 30 s. 3. Allow the suspended mixture to stand at room temperature for 15 min. 4. Centrifuge the mixture for 5 min at 1500 rpm. Discard the supernatant. B. To prepare cultures for acid-fast bacilli: 1. Suspend the pellet from Step A(4) in 5 to 10 ml of 1% sodium hydroxide by mixing on a vortex mixer for 1 min. 2. Centrifuge the mixture for 15 min at 3000 rpm. Discard the supernatant. 3. Rinse the pellet twice in 10 ml of sterile normal saline to remove the residual sodium hydroxide. 4. Resuspend the pellet in 0.5 ml of the diluted SPUTOLYSIN® Reagent.
          CAS number3483-12-3
          RTECSEK1610000
          SolubilityDilute the contents of one vial to 100 ml with sterile distilled water. Swirl gently to dissolve any crystals. If the diluted material appears cloudy, the solution may be filtered to remove any insoluble particles. The diluted solution is ~6.5 mM dithiothreitol in 100 mM phosphate buffer, pH 7.0.
          Storage +15°C to +30°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsOnce a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.
          Toxicity Harmful
          ReferencesHirsch, S.R., et al. 1969. J. Lab. Clin. Med. 74, 346.
          Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis. 94, 454.
          Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
          Cleland, W.W. 1964. Biochemistry 3, 480.
          May, J.R. 1953 (September). Lancet p. 535.
          Citation
        • I.S. Woolhouse, D.L. Bayley and R.A. Stockley. (2002) Effect of Sputum Processing with Dithiothreitol on the Detectino of Inflammatory Mediators in Chronic Bronchitis and Bronchiectasis. Thorax 57, 667-671.