03-0108-00 SMC™ Mouse TNF-alpha (TNF-α) Immunoassay Kit
IMMUNOASSAY KIT

03-0108-00
96 well assay  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      M Single Molecule Counting (SMC™)
      Description
      Catalogue Number03-0108-00
      Trade Name
      • SMC™
      DescriptionSMC™ Mouse TNF-alpha (TNF-α) Immunoassay Kit
      IMMUNOASSAY KIT
      OverviewThe SMC™ Mouse TNF-α Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
      Background InformationTumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine with cytotoxic activity that is primarily produced by macrophages. TNF-α is involved in systemic inflammation and is a member of the group of cytokines that stimulate the acute phase reaction. When administered to animals or humans it causes inflammation, fever, cardiovascular effects, hemorrhage, coagulation and acute phase responses similar to those seen during acute infections and shock states. By promoting the inflammatory response, TNF-α is associated with many autoimmune disorders such as rheumatoid arthritis, ankylosing spondylitis, Crohn’s disease, psoriasis and refractory asthma. TNF-α also plays a role in cancer and regulates apoptotic cell death, cellular proliferation, differentiation, tumorigenesis and viral replication.
      References
      Product Information
      Components
      • Mouse TNFα Coated Beads
      • Standard Diluent
      • Mouse TNFα Detection Antibody
      • Assay Buffer
      • Mouse TNFα Standard
      • SMC™ Mouse TNFα Immunoassay Kit Instructions
      • 10X Wash Buffer
      • Elution Buffer B
      • Buffer D
      Precision, %
      • Low-end CV% Range: 0 - 17%
      • Low-end CV% Average: 8%
      Quality LevelMQ200
      Applications
      ApplicationThe sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Mouse TNF-a Immunoassay Kit unparalleled performance in the quantification of TNF-α in mouse serum.
      Key Applications
      • Single Molecule Counting (SMC™)
      Biological Information
      Species Reactivity
      • Mouse
      Accuracy
      • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
      Physicochemical Information
      Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Mouse TNF-α Immunoassay Kit Instructions

      SMC™ Mouse TNF-alpha (TNF-α) Immunoassay Kit
      IMMUNOASSAY KIT SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      Singulex Erenna® Immunoassay System (MilliporeSigma)

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Singulex Erenna® single molecule counting immunoassays outperform electrochemiluminescence (ECL) methods (EMD)

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      IMMUNOASSAY KIT
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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits