03-0180-00 SMC™ Human VEGF-A High Sensitivity Immunoassay Kit

03-0180-00
96 well assay  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      NhP, Fe, Ca Single Molecule Counting (SMC™)
      Description
      Catalogue Number03-0180-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human VEGF-A High Sensitivity Immunoassay Kit
      OverviewThe SMC™ Human VEGF-A High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
      Background InformationVascular Endothelial Cell Growth Factor A (VEGF-A), one of five members of the VEGF family (VEGF-A, VEGF-B, VEGF-C, VEGF-D and Placenta Growth Factor), is a signaling protein critical in angiogenesis and vasculogenesis, predominantly acting on vascular endothelial cells. It has an important role in embryonic development and in wound healing in adults but overexpression in disease can lead to conditions such as vascular disease in the retina, tumor development and growth due to enhanced blood supply while a decrease in VEGF in pulmonary arteries is associated with pulmonary emphysema.
      References
      Product Information
      Components
      • Assay Buffer
      • VEGF Coated Beads
      • Standard Diluent
      • VEGF Detection Antibody
      • VEGF Standard
      • 10X Wash Buffer
      • Buffer D
      • Elution Buffer B
      Precision, %
      • Intra-assay CV% average (n=20): <15%
      • Inter-assay CV% average (n=5): <20%
      Applications
      ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human VEGF-A High Sensitivity Immunoassay Kit unparalleled performance in the quantification of VEGF-A in human serum and plasma.
      Key Applications
      • Single Molecule Counting (SMC™)
      Application NotesThis new streamline kit has been improved with the following features including new including new improvements over the legacy VEGF kit, #03-0068-00:
      • Improved options for sample prep and incubation
      • Lyophilization of the standard provides the kit at one storage condition
      • Removal of a plate transfer step
      • Increased reagent volumes to accommodate automation
      • Validated to run on both SMCxPRO™ and Erenna®
      Biological Information
      Species Reactivity
      • Non-human Primate
      • Feline
      • Canine
      Species Reactivity NoteAlthough VEGF was detectable in non-human primate, feline, and canine plasma, the assay has not been validated for these species.
      Cross ReactivityThe SMC™ Human VEGF-A High Sensitivity Immunoasay Kit was not found to be cross-reactive with the following:
      rhVEGF-B
      rhVEGF-C
      rhVEGF-D
      Accuracy
      • Minimum of 5 serum samples spiked with average recovery of 103% with a range of 95-110%
      • Minimum of 5 plasma samples spiked with average recovery of 93% with a range of 80-107%
      Physicochemical Information
      Sensitivity
      • Lower Limit of Quantification: 0.20 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications