03-0163-00 SMC™ Human TNFα High Sensitivity Immunoassay Kit

96 well assay  
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      Key Specifications Table

      Species ReactivityKey Applications
      NhP, R, Fe, Ca, Eq Single Molecule Counting (SMC™)
      Catalogue Number03-0163-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human TNFα High Sensitivity Immunoassay Kit
      OverviewThe SMC™ Human TNFα High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
      Background InformationTNF alpha (TNFα) is a cytokine produced by activated macrophages as well as other cells such as lymphocytes, neutrophils and mast cells. It is a key cytokine in the acute phase reaction and its primary role is regulation of immune cells. It can induce fever, inflammation and apoptotic cell death while dysregulation of TNFα has been shown to play a role in diseases such as Alzheimer’s disease, cancer, psoriasis and inflammatory bowel disease.
      Product Information
      • Assay Buffer
      • Human TNFα Beads
      • Standard Diluent
      • TNFα Detection Antibody
      • TNFα Standard
      • 10X Wash Buffer
      • Buffer D
      • Elution Buffer B
      Precision, %
      • Intra-assay CV% average (n=20): <15%
      • Inter-assay CV% average (n=5): <20%
      ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human TNFα High Sensitivity Immunoassay Kit unparalleled performance in the quantification of TNFα in human serum and plasma.
      Key Applications
      • Single Molecule Counting (SMC™)
      Application NotesThis new streamline kit has been improved with the following features while the kit performance is consistent with the legacy TNFα kit, #03-0088-00:
      • Improved options for sample prep and incubation
      • Lyophilization of the standard provides the kit at one storage condition
      • Removal of a plate transfer step
      • Overnight incubation can be used
      • Increased reagent volumes to accommodate automation
      • Validated to run on both SMCxPRO™ and Erenna®
      Biological Information
      Species Reactivity
      • Non-human Primate
      • Rat
      • Feline
      • Canine
      • Horse
      Species Reactivity NoteAlthough TNFα was detectable in non-human primate, rat, feline, canine, and equine samples, the assay has not been validated for these species.
      • Minimum of 3 serum samples spiked with average recovery of 92% with a range of 85-97%
      • Minimum of 3 plasma samples spiked with average recovery of 88% with a range of 82-91%
      Physicochemical Information
      • Lower Limit of Quantification: 0.2 pg/mL
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information