03-0057-00 SMC™ Human Interleukin 12 (IL-12)
IMMUNOASSAY KIT

03-0057-00
96 well assay  
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey Applications
      IL-12 H Single Molecule Counting (SMC™)
      Description
      Catalogue Number03-0057-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human Interleukin 12 (IL-12)
      IMMUNOASSAY KIT
      OverviewThe SMC™ Human IL-12 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
      Background InformationInterleukin-12 (IL-12) is a cytokine expressed by T-cells and natural killer cells in response to the presence of microbial agents. Increased expression of IL-12 drives a positive feedback loop, which generates more T-cells and natural killer cells, along with other immuno-regulatory cytokines such as IFN-γ, IL-10 and TNF-α. Although IL-12 is an important initiator of cell-mediated immunity, its pro-inflammatory effects can be deleterious. Over production of this cytokine has been implicated in several autoimmune disorders such as inflammatory bowel disease, psoriasis, and Crohn’s disease. The ability to escalate and strengthen the immune response makes IL-12 an important immunostimulant therapy for infectious diseases and metastatic cancers.
      References
      Product Information
      Components
      • IL-12 Coated Beads
      • Standard Diluent
      • IL-12 Detection Antibody
      • Assay Buffer
      • Human IL-12 Standard
      • SMC™ Human IL-12 Immunoassay Kit Instructions
      • 10X Wash Buffer
      • Elution Buffer B
      • Buffer D
      Precision, %
      • Low-end CV% Range: 2 - 8%
      • Low-end CV% Average: 4%
      Quality LevelMQ200
      Applications
      ApplicationThe sensitivity of Single Molecule Counting (SMC™) gives the SMC™ Human IL-12 Immunoassay Kit unparalleled performance in the quantification of IL-12 in human EDTA plasma and serum.
      Key Applications
      • Single Molecule Counting (SMC™)
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • IL-12
      Accuracy
      • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
      Physicochemical Information
      Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Erenna SMC¿ Human IL-12 Immunoassay Kit Instructions

      SMC™ Human Interleukin 12 (IL-12)
      IMMUNOASSAY KIT SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      Singulex Erenna® Immunoassay System (MilliporeSigma)

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Singulex Erenna® single molecule counting immunoassays outperform electrochemiluminescence (ECL) methods (EMD)

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits