03-0171-00 SMC™ Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit

03-0171-00
96 well assay  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Single Molecule Counting (SMC™)
      Description
      Catalogue Number03-0171-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit
      OverviewThe SMC™ Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
      Background InformationBrain-derived neurotrophic factor (BDNF) is a protein made by neuronal cells critical for the growth and functioning of neurons as well as binding at synapses to improve signal strength between neurons. While sufficient levels of BDNF promote the function and growth of brain cells and neurons, a decrease in BDNF levels can lead to impaired neurological function and is associated with diseases such as Alzheimer’s, epilepsy, schizophrenia and depression.
      References
      Product Information
      Components
      • Assay Buffer
      • BDNF Coated Beads
      • Standard Diluent
      • BDNF Detection Antibody
      • BDNF Standard
      • 10X Wash Buffer
      • Buffer D
      • Elution Buffer B
      Precision, %
      • Intra-assay CV% average (n=20): <10%
      • Inter-assay CV% average (n=5): <11%
      Applications
      ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of BDNF in human EDTA plasma, serum, and cerebrospinal fluid.
      Key Applications
      • Single Molecule Counting (SMC™)
      Application NotesThis new kit includes the following features:
      • Improved options for sample prep and incubation
      • Lyophilization of the standard provides the kit at one storage condition
      • Removal of a plate transfer step
      • Increased reagent volumes to accommodate automation
      • Validated to run on both SMCxPRO™ and Erenna®
      Biological Information
      Cross ReactivityCross reactivity and interference of ProBDNF was tested in this assay. The assay shows 1.2% cross reactivity with ProBDNF. Serum and plasma spiked with ProBDNF show an average 4.4% interference.
      Accuracy
      • Minimum of 5 EDTA plasma samples spiked with average recovery of 96% with a range of 81-108%
      • Minimum of 5 serum samples spiked with average recovery of 90% with a range of 71-98%
      • Minimum of 5 cerebrospinal fluid (CSF) samples spiked with average recovery of 98% with a range of 83-108%
      Physicochemical Information
      Sensitivity
      • Lower Limit of Quantification: 0.04 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications