71401 Rosetta™ 2(DE3)pLysS Singles™ Competent Cells - Novagen

71401
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          Description
          OverviewA common method for transformation of DNA plasmids into E. coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen prepared competent cells. Novagen® Singles™ Competent Cells are designed for ultimate convenience and reliability in plasmid transformation. Chemically competent cells are prepared using an optimized method. Cells are provided in 50µl volumes, eliminating the need to aliquot, freeze/thaw, or waste partially used vials, saving time and money and ensuring reliable cell performance. To use, simply thaw, add DNA, incubate 5 minutes on ice, heat shock for 30 seconds, place on ice for 2 minutes, and add SOC medium.
          T7 expression strains are lysogens of bacteriophage DE3, as indicated by the (DE3). These hosts carry a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in appropriate T7 expression vectors, using IPTG as an inducer.
          Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters.

          DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

          pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
          Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysSRARE2 (CamR)


          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number71401
          Brand Family Novagen®
          Features and benefits• Guaranteed transformation efficiencies:
          > 1.5 × 10⁸ cfu/µg (NovaBlue and NovaBlueT1R)
          > 2 × 10⁶ cfu/µg [BL21(DE3), BL21(DE3)pLysS, Origami™(DE3), Origami 2(DE3), Rosetta™(DE3), Rosetta 2(DE3)pLysS]
          • Provided as frozen single-use aliquots in 11- reaction or 22-reaction kits
          • Includes Test Plasmid and SOC Medium
          • Selection of popular host strains: NovaBlue for high efficiency cloning, and λDE3 lysogens for protein expression with pET vectors
          • Prepared using an optimized chemical method
          References
          Product Information
          11 rxn or 22 rxnRosetta 2(DE3)pLysS Competent Cells
          2 × 2 ml or 4 × 2 mlSOC Medium
          10 µlTest Plasmid
          Guaranteed efficiency> 2 × 10⁶ cfu/mg
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Rosetta™ 2(DE3)pLysS Singles™ Competent Cells - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          Rosetta™ 2(DE3)pLysS Singles™ Competent Cells - Novagen Certificates of Analysis

          TitleLot Number
          71401

          Brochure

          Title
          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (MilliporeSigma)

          Citations

          Title
        • Ray, D., et al. 2006. Journal of Virology 80, 8362.
        • Crosson, S., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 8018.
        • User Protocols

          Title
          TB055 pET System Manual