553506 RAP, Human, Recombinant, E. coli

553506
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553506-50UG
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      Plastic ampoule 50 μg
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      Description
      OverviewRecombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low density lipoprotein receptor-related protein (LRP) and very low-density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low density lipoprotein receptor (LDLR) and several of its class 2 mutants. Expressed as a GST-fusion protein that is subsequently cleaved with thrombin. Typical concentrations to block LRP or VLDL receptor are 200-300 nM. For antagonism of LDLR, 1 µM is recommended.
      Catalogue Number553506
      Brand Family Calbiochem®
      SynonymsReceptor Associated Protein
      References
      ReferencesLi, Y., et al. 2002. Biochemistry 41, 4921.
      Salicioni, A.M., et al. 2002. J. Biol. Chem. 277, 16160.
      Anderson, O.M., et al. 2001. Biochemistry 40, 15408.
      Bu, G. 2001. Int. Rev. Cytol. 209, 79.
      Williams, S.E., et al. 1992. J. Biol. Chem. 267, 9035
      Product Information
      FormLiquid
      Formulation150 mM NaCl, 100 mM Tris-HCl, pH 7.4.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      RAP, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      RAP, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      553506

      References

      Reference overview
      Li, Y., et al. 2002. Biochemistry 41, 4921.
      Salicioni, A.M., et al. 2002. J. Biol. Chem. 277, 16160.
      Anderson, O.M., et al. 2001. Biochemistry 40, 15408.
      Bu, G. 2001. Int. Rev. Cytol. 209, 79.
      Williams, S.E., et al. 1992. J. Biol. Chem. 267, 9035
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-April-2009 JSW
      SynonymsReceptor Associated Protein
      DescriptionRecombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Receptor Associated Protein (RAP) antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low-density lipoprotein receptor-related protein (LRP) and very low density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low-density lipoprotein receptor (LDLR) and several of its class 2 mutants. Typical concentrations used to block LRP or VLDL receptor are 200-300 nM; for antagonism of LDLR, 1 µM is recommended.
      FormLiquid
      Formulation150 mM NaCl, 100 mM Tris-HCl, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥90% by SDS-PAGE
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesLi, Y., et al. 2002. Biochemistry 41, 4921.
      Salicioni, A.M., et al. 2002. J. Biol. Chem. 277, 16160.
      Anderson, O.M., et al. 2001. Biochemistry 40, 15408.
      Bu, G. 2001. Int. Rev. Cytol. 209, 79.
      Williams, S.E., et al. 1992. J. Biol. Chem. 267, 9035