Key Specifications Table
|Description||QCM 3 µm Endothelial Cell Migration Assay - Fibronectin, Fluorometric|
|Overview||Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here
Angiogenesis is a fundamental process involving the growth of new blood vessels from pre-existing vessels. It is important in development and wound healing, as well as pathologic diseases such as diabetic retinopathy and cancer. During angiogenesis, endothelial cells need to move out of existing vessels, migrate into new areas, proliferate and assemble into new capillaries. The migration of endothelial cells is regulated by many angiogenic factors and anti-angiogenic factors. It is critical for researchers to understand the mechanisms of endothelial cell migration.
Millipore's 3 μm QCM™ Endothelial Cell Migration Assay – Fibronectin, Fluorometric provides a quick and efficient system to study the ability of compounds to induce or inhibit endothelial cell migration. This assay also allows screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for endothelial cell migration, analysis of gene function in transfected cells, and determination of ECM protein involvement in cell movement.
This versatile assay permits counting of individual migratory cells, and, more importantly, allows quantitative analysis by optical density (OD) using a standard microplate reader. This convenient assay allows large scale screening and quantitative comparison of multiple samples and includes individual migration controls for each sample.
|Materials Required but Not Delivered||1. Endothelial cells (for example: HUVEC cells), and cell culture medium (for example: EGM-2).
2. Serum-free medium, such as serum-free EGM-2 containing 0.5% BSA.
3. Sterile PBS or HBSS to wash cells.
4. Distilled water
5. (Optional) Chemoattractant or pharmacological agent for addition to culture medium.
6. Low speed centrifuge and tubes for cell harvesting
7. CO2 incubator appropriate for subject cells
8. Hemocytometer or other means of counting cells.
9. Trypan blue or equivalent viability stain
10. Fluorescence plate reader.
|Detection method||Colorimetric Fluorescent|
|Safety Information according to GHS|
|Material Size||1 kit|
|Material Package||Sufficient for 12 assays|
QCM 3 µm Endothelial Cell Migration Assay - Fibronectin, Fluorometric SDS
|Reference overview||Pub Med ID|
|Effects of lycopene and apigenin on human umbilical vein endothelial cells in vitro under angiogenic stimulation.|
Mehmet Sahin,Emel Sahin,Saadet Gümüşlü
Acta histochemica 114 2012
Angiogenesis is the formation process of new blood vessels from preexisting vessels. Solid tumors need angiogenesis for growth and metastasis. The suppression of tumor growth by inhibition of neoangiogenic processes represents a potential approach to cancer treatment. Lycopene has powerful antioxidant capacities and anticarcinogenic properties. The aim of this study was to investigate the effects of lycopene on angiogenesis in vitro. For this reason, we measured in vitro angiogenesis in human umbilical vein endothelial cells including parameters of cell proliferation, tube formation, cell migration. Lycopene and apigenin were observed to block the endothelial cell proliferation in a dose-dependent manner. In addition, they significantly decreased the capillary-like tube lengths, tube formation and endothelial cell migration. This study provides indications that apigenin and lycopene, which are considered as chemopreventive agents, to be effective in vitro on endothelial cells and angiogenesis.