508838 MilliporePuromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested - CAS 58-58-2 - Calbiochem

An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
References
Data Sheet

Synonyms: (2S)-2-Amino-N-((2S,3S,4R,5R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl)

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Overview

Replacement Information

Key Specifications Table

Empirical Formula	CAS #
C₂₂H₂₉N₇O₅ • 2HCl	58-58-2

Pricing & Availability

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Description
Overview	An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the A site of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.
Catalogue Number	508838
Brand Family	Calbiochem®
Synonyms	(2S)-2-Amino-N-((2S,3S,4R,5R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl)

References
References	Chow, S.C., et al. 1995. Exp. Cell Res. 216, 149. Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976. de la Luna, S. and Ortin, J. 1992. Methods Enzymol. 216, 376. Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.

Product Information
CAS number	58-58-2
Form	Liquid
Formulation	Supplied at 10 mg/ml in H₂O.
Hill Formula	C₂₂H₂₉N₇O₅ • 2HCl
Chemical formula	C₂₂H₂₉N₇O₅ • 2HCl
Reversible	Y
Quality Level	MQ200

Applications

Biological Information
Primary Target	RNA
Purity	≥98% by HPLC.

Physicochemical Information
Contaminants	Endotoxin: ≤10 EU/mg dry weight

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Protect from Light	Protect from light
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Packaging Information
Packaged under inert gas	Packaged under inert gas

Transport Information

Supplemental Information

Specifications

Documentation

Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested - CAS 58-58-2 - Calbiochem SDS

Title
Safety Data Sheet (SDS)

References

Reference overview
Chow, S.C., et al. 1995. Exp. Cell Res. 216, 149. Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976. de la Luna, S. and Ortin, J. 1992. Methods Enzymol. 216, 376. Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	13-May-2014 JSW
Synonyms	(2S)-2-Amino-N-((2S,3S,4R,5R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl)
Description	An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the A site of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.
Form	Liquid
Formulation	Supplied at 10 mg/ml in H₂O.
Intert gas (Yes/No)	Packaged under inert gas
CAS number	58-58-2
Chemical formula	C₂₂H₂₉N₇O₅ • 2HCl
Purity	≥98% by HPLC.
Contaminants	Endotoxin: ≤10 EU/mg dry weight
Storage	-20°C Protect from light Avoid freeze/thaw
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
Toxicity	Standard Handling
References	Chow, S.C., et al. 1995. Exp. Cell Res. 216, 149. Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976. de la Luna, S. and Ortin, J. 1992. Methods Enzymol. 216, 376. Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.