540411 Puromycin, Dihydrochloride, Cell Culture-Tested - CAS 58-58-2 - Calbiochem

Puromycin, CAS 58-58-2, is a protein synthesis inhibitor that causes premature release of nascent polypeptide chains.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: protein synthesis
540411
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₂H₂₉N₇O₅ · 2HCl 58-58-2

      Pricing & Availability

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      540411-100MG
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          540411-25MG
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              Description
              OverviewProtein synthesis inhibitor. Inhibits translation by causing premature release of nascent polypeptide chains. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
              Catalogue Number540411
              Brand Family Calbiochem®
              References
              ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
              Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
              de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
              Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.
              Product Information
              CAS number58-58-2
              FormWhite to off-white solid
              Hill FormulaC₂₂H₂₉N₇O₅ · 2HCl
              Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
              Applications
              Biological Information
              Primary Targetprotein synthesis
              Purity≥98% by HPLC
              Physicochemical Information
              ContaminantsEndotoxin: ≤10 EU/mg dry weight
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSAU7355000
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Harmful
              Storage -20°C
              Protect from Moisture Protect from moisture
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              Puromycin, Dihydrochloride, Cell Culture-Tested - CAS 58-58-2 - Calbiochem SDS

              Title

              Safety Data Sheet (SDS) 

              Puromycin, Dihydrochloride, Cell Culture-Tested - CAS 58-58-2 - Calbiochem Certificates of Analysis

              TitleLot Number
              540411

              References

              Reference overview
              Chow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
              Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
              de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
              Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.

              Brochure

              Title
              Antibiotics Profiler
              The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (EMD)
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision12-April-2011 JSW
              DescriptionAn aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the "A site" of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.
              FormWhite to off-white solid
              CAS number58-58-2
              RTECSAU7355000
              Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
              Purity≥98% by HPLC
              ContaminantsEndotoxin: ≤10 EU/mg dry weight
              SolubilityH₂O (50 mg/ml) and Methanol (20 mg/ml)
              Storage -20°C
              Protect from moisture
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
              Toxicity Harmful
              Merck USA index14, 7943
              ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
              Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
              de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
              Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.