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540411 Puromycin, Dihydrochloride, Cell Culture-Tested - CAS 58-58-2 - Calbiochem

Puromycin, CAS 58-58-2, is a protein synthesis inhibitor that causes premature release of nascent polypeptide chains.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: protein synthesis
540411
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Overview

Replacement Information

Key Specifications Table

Empirical FormulaCAS #
C₂₂H₂₉N₇O₅ · 2HCl 58-58-2

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540411-100MG
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      540411-25MG
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          Description
          OverviewProtein synthesis inhibitor. Inhibits translation by causing premature release of nascent polypeptide chains. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
          Catalogue Number540411
          Brand Family Calbiochem®
          References
          ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
          Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
          de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
          Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.
          Product Information
          CAS number58-58-2
          FormWhite to off-white solid
          Hill FormulaC₂₂H₂₉N₇O₅ · 2HCl
          Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
          Quality LevelMQ100
          Applications
          Biological Information
          Primary Targetprotein synthesis
          Purity≥98% by HPLC
          Physicochemical Information
          ContaminantsEndotoxin: ≤10 EU/mg dry weight
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSAU7355000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful
          Storage -20°C
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Puromycin, Dihydrochloride, Cell Culture-Tested - CAS 58-58-2 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Puromycin, Dihydrochloride, Cell Culture-Tested - CAS 58-58-2 - Calbiochem Certificates of Analysis

          TitleLot Number
          540411

          References

          Reference overview
          Chow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
          Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
          de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
          Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.

          Brochure

          Title
          Antibiotics Profiler
          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (MilliporeSigma)
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision12-April-2011 JSW
          DescriptionAn aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the "A site" of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.
          FormWhite to off-white solid
          CAS number58-58-2
          RTECSAU7355000
          Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
          Purity≥98% by HPLC
          ContaminantsEndotoxin: ≤10 EU/mg dry weight
          SolubilityH₂O (50 mg/ml) and Methanol (20 mg/ml)
          Storage -20°C
          Protect from moisture
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Harmful
          Merck USA index14, 7943
          ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
          Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
          de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
          Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.