Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

71049 Proteinase K Solution, 600 mAU/ml

71049
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
71049-3 Glass bottle 2 ml
71049-4 Glass bottle 10 ml
Description
OverviewProteinase K is a highly active 28,904-Da serine protease isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA. Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 µg/ml for protein removal and enzyme inactivation, and up to 2 mg/ml for tissue treatment. The Proteinase K, Lyophilized powder can be prepared as a 20 mg/ml stock solution in water and stored in aliquots at -20°C. The enzyme is also available as a ready-to-use concentrated stock solution (600 mAU/ml) that is convenient for routine use in most applications. 1 mg of Proteinase K is the equivalent of 30 mAU (AU = Anson unit). The Novagen Proteinase K products are free of detectable DNase and RNase
Catalogue Number71049
Brand Family Novagen®
References
Product Information
Unit of DefinitionOne AU (AU = Anson unit) is defined as the amount of enzyme that liberates 1.0 µmol (181 µg) of tyrosine from casein per minute at pH 7.5 at 37°C.
Quality LevelMQ400
Applications
Biological Information
Physicochemical Information
ContaminantsFree of detectable DNase and RNase.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Harmful
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
71049-3 07790788055455
71049-4 07790788053000

Documentation

Proteinase K Solution, 600 mAU/ml Certificates of Analysis

TitleLot Number
71049

Brochure

Title
High fidelity gene amplification
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

Citations

Title
  • Shannon M. Anderson, et al. (2007) New markers for murine memory B cells that define mutated and unmutated subsets. Journal of Experimental Medicine 204, 2103-2114.
  • Andrew A. Horwitz, et al. (2007) ATP-induced structural transitions in PAN, the proteasome-regulatory ATPase complex in Archaea. Journal of Biological Chemistry 282, 22921-22929.
  • User Protocols

    Title
    TB271 Proteinase K