539322 Protein G, Peroxidase Conjugate

539322
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539322-500UG
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      Plastic ampoule 500 μg
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      Description
      OverviewPurified recombinant protein G conjugated to peroxidase. Binds ≥ 99% to human IgG immobilized on a column.
      Catalogue Number539322
      Brand Family Calbiochem®
      References
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.5 mM EDTA, 10% mannose, 2% sucrose, 1% BSA, pH 7.6.
      Preservative0.02% Thimerosal
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesDirect Immunoassay (1:5000)
      Application CommentsBinds ≥99% to human IgG immobilized on a column. o-Phenylenediamine, 1 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C, is used for testing immunoenzyme reactivity Purity of the recombinant protein G has been determined by presence of a single band at M.W. 33,800 on a 15% SDS-polyacrylamide gel. Specifically binds the Fc region of IgG from various species. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      PuritySingle band at 33.8 kDa by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Mercury prohibited To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protein G, Peroxidase Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Protein G, Peroxidase Conjugate Certificates of Analysis

      TitleLot Number
      539322
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-August-2007 RFH
      ApplicationDirect Immunoassay (1:5000)
      DescriptionPurified recombinant protein G conjugated to peroxidase.
      BackgroundProtein G is a cell-surface protein of Streptococcus species that binds to the Fc portions of immunoglobulins from various species. Like protein A, it is a versatile and economic means to isolate, detect and purify antibodies produced by many animals. Protein G is especially valued because of its ability to react with Human IgG3 and a number of animal immunoglobulins with which protein A does not react well (i.e. bovine, goat, horse, rat and sheep). It also reacts more strongly with mouse immunoglobulins than protein A. Additionally, unlike protein A, it does not react with IgA, IgE and IgM.
      FormLiquid
      FormulationIn 10 mM PBS, 0.5 mM EDTA, 10% mannose, 2% sucrose, 1% BSA, pH 7.6.
      Concentration Label Please refer to vial label for lot-specific concentration
      PuritySingle band at 33.8 kDa by SDS-PAGE
      Preservative0.02% Thimerosal
      CommentsBinds ≥99% to human IgG immobilized on a column. o-Phenylenediamine, 1 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C, is used for testing immunoenzyme reactivity Purity of the recombinant protein G has been determined by presence of a single band at M.W. 33,800 on a 15% SDS-polyacrylamide gel. Specifically binds the Fc region of IgG from various species. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling