500502 Proteasome Inhibitor XXI, Z-LLLL-SF - Calbiochem

500502
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₃₂H₅₃FN₄O₇S

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      Glass bottle 5 mg
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      Description
      OverviewA cell-permeable tetrapeptidylsulfonylfluoride-based irreversible proteasome inhibitor that selectively inhibits the hydrolysis of Suc-LLVY-AMC (Cat. No. 539142) by the β5 chymotrypsin-like activity (IC50 = 7 nM using yeast 20S proteasome), while exhibiting much reduced potency against Z-LLE-AMC (Cat. No. 539141) hydrolysis by the β1 caspase-like activity or Z-LLR-AMC hydrolysis by the β2 trypsin-like activity (29% and 75% inhibition, respectively, with 200 µM inhibitor). One hour inhibitor treatment at 5 µM in HEK cultures is shown to completely abolish β5 labeling by the proteasome probe MV151, while only partial MV151 blockage is seen with β1 and β2 at an inhibitor concentration of 50 µM.

      Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
      Catalogue Number500502
      Brand Family Calbiochem®
      SynonymsCbz-Leu-Leu-Leu-Leu-ψ-[CH2SO2]-F, Cbz-Leu₄-SF, Cbz-LLLL-SF, Z-Leu₄-SF, Z-L₄-SF
      References
      ReferencesBrouwer. A.J., et al. 2013. J. Med. Chem. 55, 10995.
      Product Information
      FormWhite powder
      Hill FormulaC₃₂H₅₃FN₄O₇S
      Chemical formulaC₃₂H₅₃FN₄O₇S
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Targetβ5 chymotrypsin-like activity of 20S proteasome
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Proteasome Inhibitor XXI, Z-LLLL-SF - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Brouwer. A.J., et al. 2013. J. Med. Chem. 55, 10995.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-July-2013 JSW
      SynonymsCbz-Leu-Leu-Leu-Leu-ψ-[CH2SO2]-F, Cbz-Leu₄-SF, Cbz-LLLL-SF, Z-Leu₄-SF, Z-L₄-SF
      DescriptionA cell-permeable tetrapeptidylsulfonylfluoride-based irreversible proteasome inhibitor that selectively inhibits the hydrolysis of Suc-LLVY-AMC (Cat. No. 539142) by the β5 chymotrypsin-like activity (IC50 = 7 nM using yeast 20S proteasome; [E]0 = 0.1 nM, [S]0 = 250 µM; 15 min preincubation before substrate addition), while exhibiting much reduced potency against Z-LLE-AMC (Cat. No. 539141) hydrolysis by the β1 caspase-like activity or Z-LLR-AMC hydrolysis by the β2 trypsin-like activity (29% and 75% inhibition, respectively; 15 min preincubation with 200 µM inhibitor). One hour inhibitor treatment at 5 µM in HEK cultures is shown to completely abolish β5 labeling by the proteasome probe MV151, while only partial MV151 blockage is seen with β1 and β2 subunits at an inhibitor concentration of 50 µM.
      FormWhite powder
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₃₂H₅₃FN₄O₇S
      Structure formulaStructure formula
      Purity≥98% by HPLC
      SolubilityDMSO (100 mg/ml)
      Storage -20°C
      Protect from moisture
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesBrouwer. A.J., et al. 2013. J. Med. Chem. 55, 10995.