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539125 Protease Assay Kit

539125
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Overview

Replacement Information

Key Specifications Table

Detection Methods
Colorimetric

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539125-1KIT
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      Description
      OverviewA kit suitable for quantitative array of a wide variety of proteases in biological samples, utilizing FTC-casein as a substrate. Enzyme activities that have been detected with this assay kit include: chymotrypsin, elastase, plasminogen, PRONASE® Protease, subtilisin, thermolysin, and trypsin.
      Catalogue Number539125
      Brand Family Calbiochem®
      Materials Required but Not Delivered Trichloroacetic acid (TCA), 5%
      Spectrophotometer that can accurately measure the absorbance at 492 nm
      Pipetting devices for accurate delivery of volumes required for the assay
      Cuvettes with optical properties suitable for use at 492 nm
      A constant-temperature water bath set at 37°C
      Microfuge for generating approximately 12,000 x g
      References
      ReferencesTwining, S.S. 1984. Anal. Biochem. 143, 30.
      Wiesner, R., et al. 1982. Anal. Biochem. 121, 290.
      Product Information
      Detection methodColorimetric
      Form200 Tests
      FormatCuvette or 96-well plate
      Kit containsA Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
      Quality LevelMQ100
      Applications
      Biological Information
      Assay time3 h
      Sample TypeBiological samples
      Physicochemical Information
      Sensitivity10 ng/ml
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Storage Conditions FTC-casein should be stored at -20°C and is stable for one year from the date of purchase. FTC-casein stored at 4°C is stable for 2-3 days. Several freeze-thaw cycles will lead to a minor increase in the background fluorescence, so following initial thawing, we suggest that this reagent be divided into 5 aliquots. FTC-casein is light sensitive and should be protected from light.
      Protease positive control should be stored at -20°C. As with many proteases, trypsin is susceptible to autolysis and you may notice a reduction in trypsin activity with time, even if stored at -20°C.
      Incubation buffer and assay buffer may be stored at 4°C or -20°C.
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Kit containsA Fluorescent-Casein Substrate, Incubation Buffer, Assay Buffer Trypsin Standard, and a user protocol.
      Specifications

      Documentation

      Protease Assay Kit SDS

      Title

      Safety Data Sheet (SDS) 

      Protease Assay Kit Certificates of Analysis

      TitleLot Number
      539125

      References

      Reference overview
      Twining, S.S. 1984. Anal. Biochem. 143, 30.
      Wiesner, R., et al. 1982. Anal. Biochem. 121, 290.

      User Protocols

      Title
      User Protocol