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ProSep® Ultra Plus Chromatography Media

Designed for high throughput affinity purification of monoclonal antibodies and other proteins.

Overview

Specifications

Ordering Information

ProSep® Ultra Plus Chromatography MediaClear Sorting & Filtering Show Filter
Catalog NumberDescriptionPack UnitPack Size
175118822Prosep® Ultra Plus 2 mL Bottle 1 x 2 mL Show Pricing & Availability
175118824Prosep® Ultra Plus 10 mL Bottle 1 x 10 mL Show Pricing & Availability
175118827Prosep® Ultra Plus 100 mL Bottle 1 x 100 mL Show Pricing & Availability
175118830Prosep® Ultra Plus 1 L Bottle 1 x 1 L Show Pricing & Availability
175118833Prosep® Ultra Plus 5 L Bottle 1 x 5 L Show Pricing & Availability
175118835Prosep® Ultra Plus 10 L Bottle 1 x 10 L Show Pricing & Availability
175118834Prosep® Ultra Plus 25 L Bottle 1 x 25 L Show Pricing & Availability

Supplied as 50% slurry in 0.1M acetate buffer, pH 5.2, 1% benzyl alcohol.

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ColumnsClear Sorting & Filtering Show Filter
Catalog NumberDescription
125067MiniChrom ProSep® Ultra Plus, 1 ml Show Pricing & Availability
125076MiniChrom Column ProSep® Ultra Plus, 5 ml Show Pricing & Availability
125135RoboColumn ProSep® Ultra Plus, 0.2 ml Show Pricing & Availability
125143RoboColumn ProSep® Ultra Plus, 0.6 ml Show Pricing & Availability

Supplied in 0.1M acetate buffer, pH 5.2, 1% benzyl alcohol.

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Documentation

Brochure

Title
Biochromatography Portfolio Guide
Chromatography Purification Guide
ProSep® Ultra Plus Chromatography Media (EMD)

Technical Info

Title
Benzyl alcohol clearance from Protein A resins - EMD version
ProSep® A Chromatography Resins: Virus Clearance and Sanitization
Technical Brief - Citric acid or acetic acid? (EMD)

Data Sheet

Title
Mobius® FlexReady Solution with Smart Flexware™ Assemblies for Chromatography and TFF
ProSep® Ultra Plus Chromatography Media (MilliporeSigma)

Posters

Title
HCP clearance strategies for Protein A chromatography
Simple process strategies to increase the utilization of Protein A media in clinical manufacturing

References

Reference overviewApplication
Determining the Useful Lifetime of Chromatography Resins: Prospective Small-Scale Studies
O’Leary et al.,Biopharm, September 2001, pp 10 -18
Biopharm, September 2001, pp 10 -18  2001

Pressure-flow relationships for packed beds of compressible chromatography media at laboratory and production scale
Stickel J.J.; Fotopoulos A.
Biotechnology Progress, 17/4 (744-751), 2001  2001

Process development studies
Determining the useful lifetime chromatography resins: Prospective small-scale studies
O'Leary R.M.; Feuerhelm D.; Peers D.; Xu Y.; Blank G.S.
BioPharm ( BIOPHARM ) (United States) 2001, 14/9 (10-18)  2001

Process development studies
Affinity chromatography is vital to protein research and development
Curling JM,BIOPHARM-THE APPLIED TECHNOLOGIES OF BIOPHARMACEUTICAL DEVELOPMENT , 2001, V14, N10 (OCT), P51-52
BIOPHARM-THE APPLIED TECHNOLOGIES OF BIOPHARMACEUTICAL DEVELOPMENT , 2001, V14, N10 (OCT), P51-52  2001

Process development studies
Industrial purification of pharmaceutical antibodies: development, operation, and validation of chromatography processes.
Fahrner RL, Knudsen HL, Basey CD, Galan W, Feuerhelm D, Vanderlaan M, Blank GS.
Biotechnol Genet Eng Rev 2001;18:301-27  2001

Process development studies
Preparative Immunoaffinity Techniques
Michael Price and Kami Beyzavi; Peptide Antigens, A Practical Approach, Chapter 5
Peptide Antigens, A Practical Approach, Chapter 5  1999

Performance comparison of Protein A affinity chromatography adsorbents for purifying recombinant monoclonal antibodies
Robert L Fahrner, Duncan H Whitney, Martin Vanderlaan, Gregory S Blank.
Biotechnol. Appl. Biochem, Vol 30 121-128, 1999  1999

Performance comparison of Protein A affinity-chromatography sorbents for purifying recombinant monoclonal antibodies
Fahrner, et al.,Biotechnol. Appl. Biochem. (1999) 30, 121-128
Biotechnol. Appl. Biochem. (1999) 30, 121-128  1999

The optimal flow rate and column length for maximum production rate of Protein A affinity chromatography
R.L Fahrner, H.V. Iyer, G.S. Blank
Bioprocess Engineering 21 (1999) 287-292 - Springer-Verlag.  1999

Process development studies
Development, validation and application of an ultra-sensitive two-site enzyme immunoassay for human follistatin
LW Evans, S Muttukrishna and NP Groome
Journal of Endocrinology, 156, 275-282, 1998  1998

FAQ

QuestionAnswer
What is the difference between ProSep-vA Ultra and ProSep-vA High Capacity chromatography media?Prosep-vA Ultra uses a 700 angstrom pore size porous glass and ProSep vA High Capacity uses a 1000 Angstrom porous glass. The Prosep-vA Ultra has a greater surface area due to the smaller pore size, which allows the immobilization of a larger amount of protein A. The increased amount of protein A results in a higher capacity resin.
How do I choose between ProSep-vA Ultra versus ProSep-vA High Capacity?Prosep-vA Ultra has a higher binding capacity than ProSep-vA High Capacity - ≥ 56mg/ml vs ≥ 40 mg/ml.

ProSep-vA High Capacity has a larger pore size and may perform better when purifying fusion proteins or antibody conjugates with molecular weights above 150 kD.
What is Prosep-vA ?ProSep-vA is native protein A derived from Staphylococcus aureus which has been immobilized onto porous glass beads. ProSep-vA is available on glass of two diffferent porosities - ProSep-vA ultra (700 angstroms) and ProSep-vA High Capacity (1000 angstroms)
What is the shelf life of the Prosep-A family of protein A affinity media?One Year. Shelf life studies are ongoing and will be increased as data becomes available. Please contact Technical Service for further shelf life information.
Do you provide reguatory support?A regulatory reference document is available for ProSep-vA High Capacity and will soon be available for Prosep-vA Ultra.

As the raw materials and the chemistry used in the manufacture of ProSep-vA Ultra are the same as for ProSep-vA High Capacity , the regulators will be familiar with the product. ProSep-vA High Capacity has been and is widely used in various licensed MAb products and products in clinical trails.

User Guides

Title
Affinity Chromatography Media

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Categories

Biopharmaceutical Manufacturing > Downstream Processing > Chromatography > Affinity Chromatography