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530618 Pin1 Inhibitor, DTM - Calbiochem

Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₁₂H₂₀N₂S₃

Products

Catalog NumberPackaging Qty/Pack
5.30618.0001 Glass bottle 10 mg
Description
OverviewA cell permeable thiuram monosulfide based compound that acts as a competitive inhibitor of Pin1 (EC50 = 4.1 µM, Ki = 50 nM). Binds to the active site of the PPIase domain of Pin1 hydrophobically and blocks the active pocket. Shown to specifically inhibit peptidyl-prolyl cis/trans isomerase activity in HeLa cells. Inhibits the proliferation of HCT116 cells (EC50 = 10 µM) by arresting cell cycle at the G0 phase. Also shown to reduce the endogenous Runx2 protein levels in the MC3T3-E1 cells. Displays excellent solubility in DMSO (~100 mM).

Please note that the molecular weight for this compound is batch-specific due to variable water content.
Catalogue Number530618
Brand Family Calbiochem®
SynonymsPPIase-Parvulin Inhibitor II
References
ReferencesYoon, W-J., et al. 2013. J. Cell Physiol. 228, 2377.
Tatara, Y., et al. 2009.Biochem Biophys Res Commun. 384, 394.
Product Information
FormYellow powder
Hill FormulaC₁₂H₂₀N₂S₃
Chemical formulaC₁₂H₂₀N₂S₃
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetPin1
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
5.30618.0001 04055977260984

Documentation

Pin1 Inhibitor, DTM - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Yoon, W-J., et al. 2013. J. Cell Physiol. 228, 2377.
Tatara, Y., et al. 2009.Biochem Biophys Res Commun. 384, 394.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision30-May-2014 JSW
SynonymsPPIase-Parvulin Inhibitor II
DescriptionA cell permeable thiuram monosulfide based compound that acts as a competitive inhibitor of Pin1 (EC50 = 4.1 µM, Ki = 50 nM). Binds to the active site of the PPIase domain of Pin1 hydrophobically and blocks the active pocket. Shown to specifically inhibit peptidyl-prolyl cis/trans isomerase activity in HeLa cells. Inhibits the proliferation of HCT116 cells (EC50 = 10 µM) by arresting cell cycle at the G0 phase. Also shown to reduce the endogenous Runx2 protein levels in the MC3T3-E1 cells. Displays excellent solubility in DMSO (~100 mM).
FormYellow powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₂H₂₀N₂S₃
Purity≥98% by HPLC
SolubilityDMSO (50 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesYoon, W-J., et al. 2013. J. Cell Physiol. 228, 2377.
Tatara, Y., et al. 2009.Biochem Biophys Res Commun. 384, 394.