GF134 Pigment Epithelium Derived Factor Protein, Recombinant human

GF134
10 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesSourcePurity
      PC Human Stable BHK (baby hamster kidney) cell transfectants PEDF is purified via affinity purification from culture supernatants; Greater than 95% pure by SDS-PAGE analysis. Product may be rendered sterile by 0.22-micron filtration.
      Description
      Catalogue NumberGF134
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionPigment Epithelium Derived Factor Protein, Recombinant human
      OverviewPigment epithelium-derived factor (PEDF) was first identified as a 50 kDa secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells (Tombran-Tink et al., 1991). PEDF has been shown to have neurotrophic activity in the retina in vivo (Cayouette et al., 1999). PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells (Karakousis, et al., 2001). The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. Recently, PEDF was shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo (Dawson et al., 2001; Stellmach et al., 2001).
      Alternate Names
      • PEDF
      References
      Product Information
      PresentationProvided liquid in 20 mM sodium phosphate, pH 6.4, 0.2 M sodium chloride, and 1 mM DTT. Product has been sterile filtered to 0.22-micron.
      Quality LevelMQ100
      Applications
      ApplicationThe Pigment epithelium-derived factor (PEDF) was first identified as a 50 kDa secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells (Tombran-Tink et al., 1991).
      Key Applications
      • Positive Control
      Biological Information
      PurityPEDF is purified via affinity purification from culture supernatants; Greater than 95% pure by SDS-PAGE analysis. Product may be rendered sterile by 0.22-micron filtration.
      SourceStable BHK (baby hamster kidney) cell transfectants
      Entrez Gene Number
      Gene Symbol
      • SERPINF1
      • Serpin-F1
      • EPC-1
      • PEDF
      • PIG35
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P36955 # Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
      SIZE: 418 amino acids; 46342 Da
      SUBCELLULAR LOCATION: Secreted. Melanosome. Note=Enriched in stage I melanosomes.
      TISSUE SPECIFICITY: Retinal pigment epithelial cells and blood plasma.
      DEVELOPMENTAL STAGE: Expressed in quiescent cells.
      DOMAIN: SwissProt: P36955 The N-terminal (AA 44-121) exhibits neurite outgrowth- inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
      PTM: The N-terminus is blocked.
      SIMILARITY: Belongs to the serpin family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -70°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

      .
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Pigment Epithelium Derived Factor Protein, Recombinant human SDS

      Title

      Safety Data Sheet (SDS) 

      Pigment Epithelium Derived Factor Protein, Recombinant human Certificates of Analysis

      TitleLot Number
      RECOMBINANT HUMAN PIGMENT EPITHELIAL-DERIVED FACTOR (PEDF)3083841
      RECOMBINANT HUMAN PIGMENT EPITHELIAL-DERIVED FACTOR (PEDF) - 21133942113394
      RECOMBINANT HUMAN PIGMENT EPITHELIAL-DERIVED FACTOR (PEDF) - 23245702324570
      RECOMBINANT HUMAN PIGMENT EPITHELIAL-DERIVED FACTOR (PEDF) - 24628252462825
      RECOMBINANT HUMAN PIGMENT EPITHELIAL-DERIVED FACTOR (PEDF) - 20423242042324
      RECOMBINANT HUMAN PIGMENT EPITHELIAL-DERIVED FACTOR (PEDF) - 20731672073167
      RECOMBINANT HUMAN PIGMENT EPITHELIAL-DERIVED FACTOR (PEDF) - 21069802106980
      RECOMBINANT HUMAN PIGMENT EPITHELIAL-DERIVED FACTOR (PEDF) - 21553982155398
      RECOMBINANT HUMAN PIGMENT EPITHELIAL-DERIVED FACTOR (PEDF) - 21997422199742
      RECOMBINANT HUMAN PIGMENT EPITHELIAL-DERIVED FACTOR (PEDF) - 31847673184767

      References

      Reference overviewPub Med ID
      Effect of over-expression of pigment epithelium derived factor (PEDF) on developing retinal vasculature in the mouse
      Wong, Wai T, et al
      Mol Vis, 10:837-44 (2004)  2004

      15547489 15547489
      Localization of pigment epithelium derived factor (PEDF) in developing and adult human ocular tissues.
      Karakousis, P C, et al.
      Mol. Vis., 7: 154-63 (2001)  2001

      Show Abstract
      11438800 11438800
      Changes in the pattern of brain-derived neurotrophic factor immunoreactivity in the rat brain after acute and subchronic haloperidol treatment.
      Dawson, N M, et al.
      Synapse, 39: 70-81 (2001)  2001

      Show Abstract
      11071712 11071712
      Pigment epithelium-derived factor delays the death of photoreceptors in mouse models of inherited retinal degenerations.
      Cayouette, M, et al.
      Neurobiol. Dis., 6: 523-32 (1999)  1999

      Show Abstract
      10600408 10600408

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors