PK1010 PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb

PK1010
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M Rb Polyclonal Antibody

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PK1010-50UL
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      Description
      OverviewRecognizes the ~72 kDa Syk protein phosphorylated at Tyr525/526 in human Syk or Tyr519/520 in mouse Syk in 293T cells expressing wild-type Syk and CD8.
      Catalogue NumberPK1010
      Brand Family Calbiochem®
      Application Data
      Detection of human Syk, phosphorylated on Tyr525/526, by immunoblotting. Samples: Whole cell lysate from 293T cells transfected with wild-type Syk and CD8, left untreated (left lane) or treated with phosphatase (right lane). Primary antibody: PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb (Cat. No. PK1010) (1:1000). Detection: chemiluminescence.
      References
      ReferencesCoopman, P.J., et al. 2000. Nature 406, 742.
      Turner, M., et al. 2000. Immunol. Today 21, 148.
      Zang, J., et al. 2000. J. Biol. Chem. 275, 35442.
      Chu, D.H., et. al. 1998. Immunol. Rev. 165, 167.
      Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol. 9, 528.
      Kurosaki, T. 1997. Curr. Opin. Immunol. 9, 309.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      Positive control293T cells transfected with wild-type Syk and CD8
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (1:1000)
      Immunoblotting (1:1000)
      Application CommentsTyr525/526 represent the phosphorylation sites in human Syk, while Tyr519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr525/526 phosphorylation sites of human Syk
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PK1010

      References

      Reference overview
      Coopman, P.J., et al. 2000. Nature 406, 742.
      Turner, M., et al. 2000. Immunol. Today 21, 148.
      Zang, J., et al. 2000. J. Biol. Chem. 275, 35442.
      Chu, D.H., et. al. 1998. Immunol. Rev. 165, 167.
      Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol. 9, 528.
      Kurosaki, T. 1997. Curr. Opin. Immunol. 9, 309.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-October-2007 RFH
      ApplicationELISA (1:1000)
      Immunoblotting (1:1000)
      Application Data
      Detection of human Syk, phosphorylated on Tyr525/526, by immunoblotting. Samples: Whole cell lysate from 293T cells transfected with wild-type Syk and CD8, left untreated (left lane) or treated with phosphatase (right lane). Primary antibody: PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb (Cat. No. PK1010) (1:1000). Detection: chemiluminescence.
      DescriptionProtein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~72 kDa Syk protein phosphorylated on Tyr525/526.
      BackgroundSyk is a protein tyrosine kinase that is widely expressed and plays a key role in hematopoietic cell signaling. Syk is reported to interact with immunoreceptor tyrosine-based activation motifs (ITAMs) found in the cytoplasmic domains of immune receptors. This interaction serves to couple activated immune receptors to downstream signaling events that mediate diverse cellular responses, such as proliferation, differentiation, and phagocytosis. Syk is also reported to act as a potential tumor suppressor in human breast carcinomas. Phosphorylation of Tyr525/526, located in the activation loop of human Syk, is required for Syk activity.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr525/526 phosphorylation sites of human Syk
      IsotypeIgG
      Specieshuman, mouse
      Positive control293T cells transfected with wild-type Syk and CD8
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      PreservativeNone
      CommentsTyr525/526 represent the phosphorylation sites in human Syk, while Tyr519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Toxicity Standard Handling
      ReferencesCoopman, P.J., et al. 2000. Nature 406, 742.
      Turner, M., et al. 2000. Immunol. Today 21, 148.
      Zang, J., et al. 2000. J. Biol. Chem. 275, 35442.
      Chu, D.H., et. al. 1998. Immunol. Rev. 165, 167.
      Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol. 9, 528.
      Kurosaki, T. 1997. Curr. Opin. Immunol. 9, 309.