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566807 PhosphoDetect™ Anti-Shc/p66 (pSer³⁶) Mouse mAb (6E10)

566807
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M M Monoclonal Antibody

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566807-1SET
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      Description
      OverviewRecognizes the ~66 kDa form of Shc phosphorylated at Ser36. Does not cross-react with the non-phosphorylated form of Shc/p66 or with unrelated phosphorylation sites.
      Catalogue Number566807
      Brand Family Calbiochem®
      Application Data
      Detection of human Shc/p66, phosphorylated on Ser36, by immunoprecipitation. Samples: Timecourse of HeLa cells stimulated with EGF. Primary antibody: PhosphoDetect™ Anti-Shc/p66 (pSer36) Mouse mAb (6E10) (Cat. No. 566807) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesFoschi, M., et al. 2001. J. Biol. Chem. 276, 26640.
      Gu, H., et al. 2000. Mol. Cell. Biol. 20, 7109.
      Migliaccio, E., et al. 1997. EMBO J. 16, 706.
      Okada, S., et al. 1997. J. Biol. Chem. 272, 28042.
      Pelicci, G., et al.1992. Cell 70, 93.
      Product Information
      FormLyophilized
      Formulation100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 125 µl control protein lyophilized from 2X PBS.
      Preservative≤0.1% sodium azide (antibody only)
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (0.05 µg/ml)
      Immunoblotting (0.1-1 µg/ml)
      Application CommentsFor immunoblotting using the p66/Shc positive control, use 15 µl per lane for colorimetric or chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁶ phosphorylation site of human Shc/p66
      Clone6E10
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 1 ml dH₂O for at least 15 min at room temperature until completely dissolved. Following reconstitution, aliquot and freeze (-20°C). Reconstitute the positive control with 125 µl H₂O until completely dissolved. After complete solubilization, add 125 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-Shc/p66 (pSer³⁶) Mouse mAb (6E10) Certificates of Analysis

      TitleLot Number
      566807

      References

      Reference overview
      Foschi, M., et al. 2001. J. Biol. Chem. 276, 26640.
      Gu, H., et al. 2000. Mol. Cell. Biol. 20, 7109.
      Migliaccio, E., et al. 1997. EMBO J. 16, 706.
      Okada, S., et al. 1997. J. Biol. Chem. 272, 28042.
      Pelicci, G., et al.1992. Cell 70, 93.

      Citations

      Title
    • Sun, L., et al. 2010. Am J Physiol Renal Physiol. 299, F1014.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-August-2007 RFH
      ApplicationELISA (0.05 µg/ml)
      Immunoblotting (0.1-1 µg/ml)
      Application Data
      Detection of human Shc/p66, phosphorylated on Ser36, by immunoprecipitation. Samples: Timecourse of HeLa cells stimulated with EGF. Primary antibody: PhosphoDetect™ Anti-Shc/p66 (pSer36) Mouse mAb (6E10) (Cat. No. 566807) (1 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified mouse monoclonal antibody. Recognizes the Shc/p66 protein phosphorylated on Ser36. Supplied with a positive control consisting of Shc/p66 from EGF-stimulated HepG2 cells immunoprecipitated using anti-Shc non-phospho-specific antibody.
      BackgroundMammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: Shc/p46, Shc/p52 and Shc/p66. Shc/p46 and Shc/p52 are found in every cell type, whereas Shc/p66 expression varies in different tissues. Shc proteins are phosphorylated by receptor tyrosine kinases and interact with phosphotyrosine residues on these receptors via the shc PTB and/or SH2 domains. The major tyrosine phosphorylation sites are tyrosines 239/240 as well as tyrosine 317. The Shc/p66 protein contains an additional N-terminal protein domain that is phosphorylated at serine 36. It is suggested that only the serine-phosphorylated Shc/p66 can interact with GRB2. Moreover, expression of Shc/p66 accelerates the inactivation of MAP kinases after EGF stimulation, whereas Shc/p46 and Shc/p52 enhance MAP kinase activity.
      HostMouse
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁶ phosphorylation site of human Shc/p66
      Clone6E10
      IsotypeIgG₁
      Specieshuman, mouse
      FormLyophilized
      Formulation100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 125 µl control protein lyophilized from 2X PBS.
      Preservative≤0.1% sodium azide (antibody only)
      CommentsFor immunoblotting using the p66/Shc positive control, use 15 µl per lane for colorimetric or chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 1 ml dH₂O for at least 15 min at room temperature until completely dissolved. Following reconstitution, aliquot and freeze (-20°C). Reconstitute the positive control with 125 µl H₂O until completely dissolved. After complete solubilization, add 125 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesFoschi, M., et al. 2001. J. Biol. Chem. 276, 26640.
      Gu, H., et al. 2000. Mol. Cell. Biol. 20, 7109.
      Migliaccio, E., et al. 1997. EMBO J. 16, 706.
      Okada, S., et al. 1997. J. Biol. Chem. 272, 28042.
      Pelicci, G., et al.1992. Cell 70, 93.
      Citation
    • Sun, L., et al. 2010. Am J Physiol Renal Physiol. 299, F1014.
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