442685 Sigma-AldrichPhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Rabbit pAb

This PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 Rabbit pAb is validated for use in Dot Blot, WB for the detection of MAP Kinase ERK1/ERK2 .

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
Brochures
Data Sheet

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Overview

Replacement Information

Key Specifications Table

Species Reactivity	Host	Antibody Type
H, R	Rb	Polyclonal Antibody

Pricing & Availability

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Description
Overview	Recognizes the ~42-44 kDa ERK1 and ERK2 proteins phosphorylated at Thr²⁰² and Tyr²⁰⁴.
Catalogue Number	442685
Brand Family	Calbiochem®
Application Data	Detection of human MAP Kinase ERK1/ERK2, phosphorylated on Thr²⁰²/Tyr²⁰⁴, by immunoblotting. Samples: Whole cell lysate from human T47D cells untreated (lane 1) or treated with lambda-phosphatase (lane 2). Primary antibody: PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Rabbit pAb (Cat. No. 442685) (1:1000). Detection: chemiluminescence.

References

Product Information
Form	Liquid
Formulation	In 150 mM NaCl, 10 mM HEPES, 100 μg/ml BSA, 50% glycerol, pH 7.5.
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Dot Blot Immunoblotting (Western Blotting)
Application Notes	Dot Blot (1:1000) Immunoblotting (1:1000)
Application Comments	Does not react with the unphosphorylated proteins. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	a synthetic phosphopeptide surrounding the Thr²⁰²/Tyr²⁰⁴ phosphorylation sites of human ERK1/ERK2
Immunogen	Human
Host	Rabbit
Isotype	IgG
Species Reactivity	Human Rat
Antibody Type	Polyclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Documentation

PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Rabbit pAb SDS

Title
Safety Data Sheet (SDS)

PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Rabbit pAb Certificates of Analysis

Title	Lot Number
442685	Enter Lot Number

Brochure

Title
MAPK Pathway Poster ( 750 KB )

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	07-August-2007 RFH
Application	Dot Blot (1:1000) Immunoblotting (1:1000)
Application Data	Detection of human MAP Kinase ERK1/ERK2, phosphorylated on Thr²⁰²/Tyr²⁰⁴, by immunoblotting. Samples: Whole cell lysate from human T47D cells untreated (lane 1) or treated with lambda-phosphatase (lane 2). Primary antibody: PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Rabbit pAb (Cat. No. 442685) (1:1000). Detection: chemiluminescence.
Description	Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~42-44 kDa ERK1 and ERK2 proteins phosphorylated at Thr²⁰² and Tyr²⁰⁴.
Host	Rabbit
Immunogen species	Human
Immunogen	a synthetic phosphopeptide surrounding the Thr²⁰²/Tyr²⁰⁴ phosphorylation sites of human ERK1/ERK2
Isotype	IgG
Species	human, rat
Form	Liquid
Formulation	In 150 mM NaCl, 10 mM HEPES, 100 μg/ml BSA, 50% glycerol, pH 7.5.
Preservative	None
Comments	Does not react with the unphosphorylated proteins. Variables associated with assay conditions will dictate the proper working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling

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