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442706 PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Mouse mAb (12D4)

442706
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R M Monoclonal Antibody

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442706-1SET
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      Description
      OverviewRecognizes the ~42-44 kDa ERK1 and ERK2 proteins phosphorylated at Thr202 and Tyr204 in vanadate-treated A431 cells. Supplied with a control lysate.
      Catalogue Number442706
      Brand Family Calbiochem®
      SynonymsAnti-MAPK1/2, Activated
      References
      Product Information
      FormLyophilized
      Formulation100 µg antibody lyophilized from 2X PBS, sucrose, polyethylene glycol (PEG) and 150 µl lyophilized control lysate from 1 x 10⁶ vanadate-treated A431 cells.
      Positive controlvanadate-treated A431 cells (supplied)
      Preservative≤0.1% sodium azide (antibody only)
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric or 0.1 µg/ml, chemiluminescence)
      Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
      Application CommentsAntibody requires phosphorylation at both the Thr and Tyr site for recognition. Does not recognize non-phosphorylated MAP kinase 1/2. Does not cross-react with SAPK 1 or SAPK2 proteins. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr204/204 phosphorylation stie of mouse ERK1 and ERK2
      ImmunogenHuman
      Clone12D4
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute antibody with 1 ml dH₂O. Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute the positive control cell lysate with 200 µl dH₂O. Following complete solubilization add 200 µl 2X SDS-PAGE sample buffer and incubate at 90°C for 5 min. Aliquot and freeze (-20°C). Avoid freeze/thaw cycles of reconstituted antibody and control lysate.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Mouse mAb (12D4) Certificates of Analysis

      TitleLot Number
      442706

      Brochure

      Title
      MAPK Pathway Poster ( 750 KB )
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2007 RFH
      SynonymsAnti-MAPK1/2, Activated
      ApplicationELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric or 0.1 µg/ml, chemiluminescence)
      Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
      DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~42-44 kDa ERK1 and ERK2 protein phosphorylated on Thr202/Tyr204. Supplied with control lysate consisting of 1 x 106 vanadate-treated A431 cells.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr204/204 phosphorylation stie of mouse ERK1 and ERK2
      Clone12D4
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlvanadate-treated A431 cells (supplied)
      FormLyophilized
      Formulation100 µg antibody lyophilized from 2X PBS, sucrose, polyethylene glycol (PEG) and 150 µl lyophilized control lysate from 1 x 10⁶ vanadate-treated A431 cells.
      Preservative≤0.1% sodium azide (antibody only)
      CommentsAntibody requires phosphorylation at both the Thr and Tyr site for recognition. Does not recognize non-phosphorylated MAP kinase 1/2. Does not cross-react with SAPK 1 or SAPK2 proteins. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute antibody with 1 ml dH₂O. Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute the positive control cell lysate with 200 µl dH₂O. Following complete solubilization add 200 µl 2X SDS-PAGE sample buffer and incubate at 90°C for 5 min. Aliquot and freeze (-20°C). Avoid freeze/thaw cycles of reconstituted antibody and control lysate.
      Toxicity Standard Handling

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