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382159 PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb

382159
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Nem, Dr, H, T, Xn Rb Polyclonal Antibody

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      Description
      OverviewRecognizes the ~17 kDa histone H3 protein phosphorylated at Ser10.
      Catalogue Number382159
      Brand Family Calbiochem®
      Application Data
      Detection of human histone H3, phosphorylated on Ser10, by immunoprecipitation. Sample: Acid extracted proteins from HeLa cells. Primary antibody: PhosphoDetect™ Anti-Histone H3 (pSer10) (7-20) Rabbit pAb (Cat. No. 382159) (assay dependent). Detection: chemiluminescence.
      References
      ReferencesAjiro, K., et al. 1996. J. Biol. Chem. 271, 13197.
      Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098.
      Mahadevan, L.C., et al. 1991. Cell 65, 775.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
      Positive controlAcid-extracted proteins from colcemid-arrested HeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoprecipitation
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunohistochemistry
      Application NotesImmunoblotting (0.5 µg/ml)
      Immunocytochemistry (5 µg/ml)
      Immunohistochemistry (see comments)
      Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
      Application CommentsFor immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Nematode
      • Drosophila
      • Human
      • Tetrahymena
      • Xenopus
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb Certificates of Analysis

      TitleLot Number
      382159

      References

      Reference overview
      Ajiro, K., et al. 1996. J. Biol. Chem. 271, 13197.
      Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098.
      Mahadevan, L.C., et al. 1991. Cell 65, 775.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-August-2007 RFH
      ApplicationImmunoblotting (0.5 µg/ml)
      Immunocytochemistry (5 µg/ml)
      Immunohistochemistry (see comments)
      Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
      Application Data
      Detection of human histone H3, phosphorylated on Ser10, by immunoprecipitation. Sample: Acid extracted proteins from HeLa cells. Primary antibody: PhosphoDetect™ Anti-Histone H3 (pSer10) (7-20) Rabbit pAb (Cat. No. 382159) (assay dependent). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser10.
      BackgroundHistone H1 and H3 phosphorylations correlate with the process of chromosome condensation. H3 mRNA-positive cells are most often found in tissues with a high proliferation rate, such as basal cells of the skin and crypt cells of the intestine.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH
      IsotypeIgG
      SpeciesC. elegans, Drosophila, Tetrahymena, Xenopus, human
      Positive controlAcid-extracted proteins from colcemid-arrested HeLa cells
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesAjiro, K., et al. 1996. J. Biol. Chem. 271, 13197.
      Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098.
      Mahadevan, L.C., et al. 1991. Cell 65, 775.

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