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ST1029 PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4)

ST1029
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

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      Description
      OverviewRecognizes the ~55 kDa c-Fos protein phosphorylated at Ser374. Does not detect the unphosphorylated form.
      Catalogue NumberST1029
      Brand Family Calbiochem®
      Application Data
      Detection of human Fos, phosphorylated on Ser374, by immunoblotting. Samples: Serum starved HepG2 cells incubated with EGF for 0 min (lane 1), 5 min (lane 2), 15 min (lane 3), 30 min (lane 4), 1 h (lane 5), 2 h (lane 6), 4 h (lane 7), and 8 h (lane 8). Primary antibody: PhosphoDetect™ Anti-Fos (pSer374) Mouse mAb (34E4) (Cat. No. ST1029) (0.5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesChen, R-H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10952.
      Product Information
      FormLyophilized
      Formulation100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate from EGF-treated HEPG2 cells.
      Positive controlHepG2 cells treated with EGF
      Preservative≤0.1% sodium azide (antibody only)
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (0.1 µg/ml)
      Immunoblotting (0.5 µg/ml, chemiluminescence)
      Application CommentsDoes not detect the unphosphorylated form of c-fos. For immunoblotting use 20 µl control lysate per lane (minigel) for chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁷⁴ phosphorylation site of human c-Fos
      ImmunogenHuman
      Clone34E4
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsReconstitute antibody with 1 ml water (15 minutes, room temperature). Following reconstitution, aliquot and freeze in liquid nitrogen. Reconstituted antibody can be stored at -80°C for up to 1 year. Aliquots may be stored at 4°C for up to 3 months and should be thawed at 37°C. Reconstitute control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconsitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4) SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4) Certificates of Analysis

      TitleLot Number
      ST1029

      References

      Reference overview
      Chen, R-H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10952.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-October-2007 RFH
      ApplicationELISA (0.1 µg/ml)
      Immunoblotting (0.5 µg/ml, chemiluminescence)
      Application Data
      Detection of human Fos, phosphorylated on Ser374, by immunoblotting. Samples: Serum starved HepG2 cells incubated with EGF for 0 min (lane 1), 5 min (lane 2), 15 min (lane 3), 30 min (lane 4), 1 h (lane 5), 2 h (lane 6), 4 h (lane 7), and 8 h (lane 8). Primary antibody: PhosphoDetect™ Anti-Fos (pSer374) Mouse mAb (34E4) (Cat. No. ST1029) (0.5 µg/ml). Detection: chemiluminescence.
      DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Supplied with a positive control lysate consisting of EGF-treated HepG2 cells. Recognizes the ~55 kDa c-fos protein phosphorylated at Ser374 by MAP kinase.
      BackgroundThe early gene product c-Fos is expressed following mitogenic stimulation and functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at Ser374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at Thr325.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁷⁴ phosphorylation site of human c-Fos
      Clone34E4
      IsotypeIgG₁
      Specieshuman
      Positive controlHepG2 cells treated with EGF
      FormLyophilized
      Formulation100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate from EGF-treated HEPG2 cells.
      Preservative≤0.1% sodium azide (antibody only)
      CommentsDoes not detect the unphosphorylated form of c-fos. For immunoblotting use 20 µl control lysate per lane (minigel) for chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute antibody with 1 ml water (15 minutes, room temperature). Following reconstitution, aliquot and freeze in liquid nitrogen. Reconstituted antibody can be stored at -80°C for up to 1 year. Aliquots may be stored at 4°C for up to 3 months and should be thawed at 37°C. Reconstitute control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconsitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Toxicity Irritant
      ReferencesChen, R-H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10952.

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