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ST1029 PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
ST1029-1SET Plastic ampoule 1 set
Description
OverviewRecognizes the ~55 kDa c-Fos protein phosphorylated at Ser374. Does not detect the unphosphorylated form.
Catalogue NumberST1029
Brand Family Calbiochem®
Application Data
Detection of human Fos, phosphorylated on Ser374, by immunoblotting. Samples: Serum starved HepG2 cells incubated with EGF for 0 min (lane 1), 5 min (lane 2), 15 min (lane 3), 30 min (lane 4), 1 h (lane 5), 2 h (lane 6), 4 h (lane 7), and 8 h (lane 8). Primary antibody: PhosphoDetect™ Anti-Fos (pSer374) Mouse mAb (34E4) (Cat. No. ST1029) (0.5 µg/ml). Detection: chemiluminescence.
References
ReferencesChen, R-H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10952.
Product Information
FormLyophilized
Formulation100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate from EGF-treated HEPG2 cells.
Positive controlHepG2 cells treated with EGF
Preservative≤0.1% sodium azide (antibody only)
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (0.1 µg/ml)
Immunoblotting (0.5 µg/ml, chemiluminescence)
Application CommentsDoes not detect the unphosphorylated form of c-fos. For immunoblotting use 20 µl control lysate per lane (minigel) for chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁷⁴ phosphorylation site of human c-Fos
ImmunogenHuman
Clone34E4
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsReconstitute antibody with 1 ml water (15 minutes, room temperature). Following reconstitution, aliquot and freeze in liquid nitrogen. Reconstituted antibody can be stored at -80°C for up to 1 year. Aliquots may be stored at 4°C for up to 3 months and should be thawed at 37°C. Reconstitute control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconsitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ST1029-1SET 04055977208689

Documentation

PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4) SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4) Certificates of Analysis

TitleLot Number
ST1029

References

Reference overview
Chen, R-H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10952.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-October-2007 RFH
ApplicationELISA (0.1 µg/ml)
Immunoblotting (0.5 µg/ml, chemiluminescence)
Application Data
Detection of human Fos, phosphorylated on Ser374, by immunoblotting. Samples: Serum starved HepG2 cells incubated with EGF for 0 min (lane 1), 5 min (lane 2), 15 min (lane 3), 30 min (lane 4), 1 h (lane 5), 2 h (lane 6), 4 h (lane 7), and 8 h (lane 8). Primary antibody: PhosphoDetect™ Anti-Fos (pSer374) Mouse mAb (34E4) (Cat. No. ST1029) (0.5 µg/ml). Detection: chemiluminescence.
DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Supplied with a positive control lysate consisting of EGF-treated HepG2 cells. Recognizes the ~55 kDa c-fos protein phosphorylated at Ser374 by MAP kinase.
BackgroundThe early gene product c-Fos is expressed following mitogenic stimulation and functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at Ser374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at Thr325.
HostMouse
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁷⁴ phosphorylation site of human c-Fos
Clone34E4
IsotypeIgG₁
Specieshuman
Positive controlHepG2 cells treated with EGF
FormLyophilized
Formulation100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate from EGF-treated HEPG2 cells.
Preservative≤0.1% sodium azide (antibody only)
CommentsDoes not detect the unphosphorylated form of c-fos. For immunoblotting use 20 µl control lysate per lane (minigel) for chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute antibody with 1 ml water (15 minutes, room temperature). Following reconstitution, aliquot and freeze in liquid nitrogen. Reconstituted antibody can be stored at -80°C for up to 1 year. Aliquots may be stored at 4°C for up to 3 months and should be thawed at 37°C. Reconstitute control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconsitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Toxicity Irritant
ReferencesChen, R-H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10952.