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Description
Overview
A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but stable over wide range of pH and temperature. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units.
Catalogue Number
53702
Brand Family
Calbiochem®
References
References
Narahashi, Y., et al. 1968. J. Biochem. 64, 427.
Product Information
CAS number
9036-06-0
Activity
≥45,000 proteolytic units/g dry weight
Unit of Definition
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
Form
Lyophilized
Formulation
Lyophilized from a solution containing calcium acetate as a stabilizer.
Hygroscopic
Hygroscopic
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECS
UK9595000
Safety Information
R Phrase
R: 20-36/37/38-42/43
Harmful by inhalation. Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation and skin contact.
S Phrase
S: 22-26-36
Do not breathe dust. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Harmful
Storage
+2°C to +8°C
Hygroscopic
Hygroscopic
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.
PRONASE® Protease, Streptomyces griseus Certificates of Analysis
Title
Lot Number
53702
References
Reference overview
Narahashi, Y., et al. 1968. J. Biochem. 64, 427.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
17-September-2007 RFH
Description
A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.
Form
Lyophilized
Formulation
Lyophilized from a solution containing calcium acetate as a stabilizer.
CAS number
9036-06-0
RTECS
UK9595000
Activity
≥45,000 proteolytic units/g dry weight
Unit definition
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
Solubility
H₂O (10 mg/ml)
Storage
+2°C to +8°C
Hygroscopic
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.
