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53702 PRONASE® Protease, Streptomyces griseus

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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53702
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
53702-10KU Glass bottle 10 ku
53702-250KU Glass bottle 250 ku
53702-25KU Glass bottle 25 ku
53702-500KU Glass bottle 500 ku
53702-50KU Glass bottle 50 ku
53702-KU Glass bottle
Description
OverviewA non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but stable over wide range of pH and temperature. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units.
Catalogue Number53702
Brand Family Calbiochem®
References
ReferencesNarahashi, Y., et al. 1968. J. Biochem. 64, 427.
Product Information
CAS number9036-06-0
Activity≥45,000 proteolytic units/g dry weight
Unit of DefinitionOne unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
FormLyophilized
FormulationLyophilized from a solution containing calcium acetate as a stabilizer.
Hygroscopic Hygroscopic
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSUK9595000
Safety Information
R PhraseR: 20-36/37/38-42/43

Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 22-26-36

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

PRONASE® Protease, Streptomyces griseus SDS

Title

Safety Data Sheet (SDS) 

PRONASE® Protease, Streptomyces griseus Certificates of Analysis

TitleLot Number
53702

References

Reference overview
Narahashi, Y., et al. 1968. J. Biochem. 64, 427.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-September-2007 RFH
DescriptionA non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.
FormLyophilized
FormulationLyophilized from a solution containing calcium acetate as a stabilizer.
CAS number9036-06-0
RTECSUK9595000
Activity≥45,000 proteolytic units/g dry weight
Unit definitionOne unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
SolubilityH₂O (10 mg/ml)
Storage +2°C to +8°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.
Toxicity Harmful
ReferencesNarahashi, Y., et al. 1968. J. Biochem. 64, 427.