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539568 PP2B, Human, Recombinant, E. coli

539568
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539568-5000U
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      Description
      OverviewRecombinant, human calcineurin Aα (catalytic subunit) and calcineurin B (regulatory subunit) co-expressed with yeast myristoyl-CoA:protein N-myristoyltransferase. A Ca2+/CaM-dependent protein phosphatase. The resulting highly active calcineurin is N-myristoylated on the CaN B-subunit, as is the native protein. M.W. 60,000 (A subunit) and 15,000 (B subunit).
      Catalogue Number539568
      Brand Family Calbiochem®
      SynonymsCaN, Protein Phosphatase 2B, Calcineurin
      References
      ReferencesGuerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
      Mondragon, A., et al. 1997. Biochemistry 36, 4934.
      Wei, Q. and Lee, E.Y. 1997. Biochemistry 36, 7418.
      Product Information
      Activity≥50,000 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1.0 pmol of phosphate from the RII phosphopeptide substrate (DLDVPIPGRFDRRVpSVAAE; <a href ="/Products/ProductDisplay.asp→catNO=207008">Cat. No. 207008</a>) in the presence of calmodulin per min at 30°C, pH 7.4.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM Tris, 6 mM MgCl₂, 5 mM DTT, 0.5 mM CaCl₂, 0.025% NP-40.
      Quality LevelMQ100
      Applications
      Biological Information
      PurityTwo distinct bands by SDS-PAGE
      Specific Activity≥300,000 units/mg
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Please refer to vial label for lot-specific reconstitution volume.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PP2B, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      PP2B, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      539568

      References

      Reference overview
      Guerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
      Mondragon, A., et al. 1997. Biochemistry 36, 4934.
      Wei, Q. and Lee, E.Y. 1997. Biochemistry 36, 7418.

      Brochure

      Title
      Protein Phosphatases Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-August-2011 JSW
      SynonymsCaN, Protein Phosphatase 2B, Calcineurin
      DescriptionRecombinant, human calcineurin Aα (catalytic subunit) and calcineurin B (regulatory subunit) co-expressed with yeast myristoyl-CoA:protein N-myristoyltransferase. A Ca2+/CaM-dependent protein phosphatase. The resulting highly active calcineurin is N-myristoylated on the CaN B-subunit, as is the native protein. M.W. 60,000 (A subunit) and 15,000 (B subunit).
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM Tris, 6 mM MgCl₂, 5 mM DTT, 0.5 mM CaCl₂, 0.025% NP-40.
      PurityTwo distinct bands by SDS-PAGE
      Specific activity≥300,000 units/mg
      Activity≥50,000 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will release 1.0 pmol of phosphate from the RII phosphopeptide substrate (DLDVPIPGRFDRRVpSVAAE; Cat. No. 207008) in the presence of calmodulin per min at 30°C, pH 7.4.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Please refer to vial label for lot-specific reconstitution volume.
      Toxicity Standard Handling
      ReferencesGuerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
      Mondragon, A., et al. 1997. Biochemistry 36, 4934.
      Wei, Q. and Lee, E.Y. 1997. Biochemistry 36, 7418.