476480 PKC 20-28, Cell-Permeable, Myristoylated - Calbiochem

476480
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₆₀H₁₀₆N₁₈O₁₁

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476480-500UG
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      Plastic ampoule 500 μg
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      Description
      OverviewPseudosubstrate sequence from protein kinase Cα and β ( PKCα and PKCβ). N-Terminus is myristoylated to allow membrane permeability. Highly specific, reversible, and substrate competitive inhibitor of TPA activation of MARCKS phosphorylation in fibroblast primary cultures (IC50 = 8 µM); exhibits 98% inhibition at 100 µM.
      Catalogue Number476480
      Brand Family Calbiochem®
      SynonymsMyristoylated Protein Kinase C Inhibitor 20-28, Cell-Permeable, Myr-N-FARKGALRQ-NH₂, Protein Kinase C 20-28, Cell-Permeable, Myristoylated
      References
      ReferencesEichholtz, T., et al. 1993. J. Biol. Chem. 268, 1982.
      Ward, N.E. and O’Brian, C.A. 1993. Biochemistry 32, 11903.
      Product Information
      ATP CompetitiveN
      FormLyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₆₀H₁₀₆N₁₈O₁₁
      Chemical formulaC₆₀H₁₀₆N₁₈O₁₁
      Hygroscopic Hygroscopic
      ReversibleY
      Sold on the basis of peptide contentY
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetTPA activation of MARCKS phosphorylation in fibroblast primary cultures
      Primary Target IC<sub>50</sub>8 µM against TPA activation of MARCKS phosphorylation in fibroblast primary cultures
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableY
      Peptide ContentY
      Peptide SequenceN-Myr-Phe-Ala-Arg-Lys-Gly-Ala-Leu-Arg-Gln-NH₂
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PKC 20-28, Cell-Permeable, Myristoylated - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      PKC 20-28, Cell-Permeable, Myristoylated - Calbiochem Certificates of Analysis

      TitleLot Number
      476480

      References

      Reference overview
      Eichholtz, T., et al. 1993. J. Biol. Chem. 268, 1982.
      Ward, N.E. and O’Brian, C.A. 1993. Biochemistry 32, 11903.

      Brochure

      Title
      PKC Pathway Poster PDF ( 676 KB )

      Citations

      Title
    • Ying Zhang, Mingjuan Liao and Maria L. Dufau. (2006) Phosphatidylinositol 3-kinase/protein kinase Cγ-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated depression of transcription of the luteinizing hormone receptor gene. Molecular and Cellular Biology 26, 6748-6761.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-January-2010 RFH
      SynonymsMyristoylated Protein Kinase C Inhibitor 20-28, Cell-Permeable, Myr-N-FARKGALRQ-NH₂, Protein Kinase C 20-28, Cell-Permeable, Myristoylated
      DescriptionPseudosubstrate sequence from protein kinase Ca (PKCα) and PKCβ. N-terminal myristoylated to allow membrane permeability. Highly specific, reversible, and substrate competitive inhibitor of TPA activation of MARCKS phosphorylation in fibroblast primary cultures (IC50 = 8 µM). Exhibits 98% inhibition at 100 µM.
      FormLyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Chemical formulaC₆₀H₁₀₆N₁₈O₁₁
      Peptide SequenceN-Myr-Phe-Ala-Arg-Lys-Gly-Ala-Leu-Arg-Gln-NH₂
      Purity≥95% by HPLC
      SolubilityTris-HCl, pH 7.5 (25 mg/ml) or H₂O (10 mg/ml)
      Storage -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
      Toxicity Standard Handling
      ReferencesEichholtz, T., et al. 1993. J. Biol. Chem. 268, 1982.
      Ward, N.E. and O’Brian, C.A. 1993. Biochemistry 32, 11903.
      Citation
    • Ying Zhang, Mingjuan Liao and Maria L. Dufau. (2006) Phosphatidylinositol 3-kinase/protein kinase Cγ-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated depression of transcription of the luteinizing hormone receptor gene. Molecular and Cellular Biology 26, 6748-6761.