Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

539481 PKA, Catalytic Subunit, Mouse, Recombinant, E. coli

View Products on Sigmaaldrich.com
539481
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
539481-100KU Plastic ampoule 100 ku
Description
OverviewRecombinant, mouse PKA catalytic subunit expressed in E. coli. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Suitable for studying the phosphorylation of purified substrates and for use with cell extracts. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.
Catalogue Number539481
Brand Family Calbiochem®
SynonymscAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
References
ReferencesGrant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
Adams, J.A., et al. 1995. Biochemistry 34, 2447.
Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
Brindle, P., et al. 1993. Nature 364, 821.
Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
Wang, L-Y., et al. 1993. Science 259, 1173.
Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.
Product Information
Activity≥2.5 x 10⁶ U/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; <a href ="/Products/ProductDisplay.asp→catNO=05-23-4900">Cat. No. 05-23-4900</a>) per min at 30°C, pH 7.5.
EC number2.7.1.37
FormLiquid
FormulationIn 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
ContaminantsPhosphatases, proteases: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
539481-100KU 04055977269178

Documentation

PKA, Catalytic Subunit, Mouse, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

PKA, Catalytic Subunit, Mouse, Recombinant, E. coli Certificates of Analysis

TitleLot Number
539481

References

Reference overview
Grant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
Adams, J.A., et al. 1995. Biochemistry 34, 2447.
Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
Brindle, P., et al. 1993. Nature 364, 821.
Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
Wang, L-Y., et al. 1993. Science 259, 1173.
Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.

Citations

Title
  • Gerald Wilson, et al. (2003) Phosphorylation of p40AUF1 regulates binding to A + U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein structure.. Journal of Biological Chemistry 278, (33039- 33048).
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision02-January-2008 JSW
    SynonymscAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
    DescriptionRecombinant, mouse PKA catalytic subunit expressed in E. coli. The catalytic subunit of protein kinase A that combines with the regulatory subunit, in the absence of cAMP, to form the inactive PKA holoenzyme. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation. PKA-Cα is reported to potentiate IP3-induced Ca2+ mobilization in isolated hepatocytes. Suitable for studies of PKA phosphorylation in vitro with purified substrates or following the introduction into intact cells or cell extracts.
    FormLiquid
    FormulationIn 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.
    Recommended reaction conditions50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 200 µM ATP, and γ-labeled-32P-ATP to a final specific activity of 200 μCi/μmol. Incubate at 30°C.
    EC number2.7.1.37
    ContaminantsPhosphatases, proteases: none detected
    Activity≥2.5 x 10⁶ U/ml
    Unit definitionOne unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; Cat. No. 05-23-4900) per min at 30°C, pH 7.5.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesGrant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022.
    Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553.
    Adams, J.A., et al. 1995. Biochemistry 34, 2447.
    Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242.
    Brindle, P., et al. 1993. Nature 364, 821.
    Hajnoczky, G., et al. 1993. Biochem. J. 293, 413.
    Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135.
    Wang, L-Y., et al. 1993. Science 259, 1173.
    Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940.
    Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300.
    Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763.
    Citation
  • Gerald Wilson, et al. (2003) Phosphorylation of p40AUF1 regulates binding to A + U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein structure.. Journal of Biological Chemistry 278, (33039- 33048).