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33-040 PI 3-Kinase 3-step HTRF™ Assay

384 wells  Kit capacity: 384 wells
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      Key Specifications Table

      Species ReactivityKey ApplicationsAnalytes AvailableDetection Methods
      H, MKAPI 3-Kinase HTRF
      Catalogue Number33-040
      Brand Family Upstate
      Trade Name
      • HTRF
      • Upstate
      DescriptionPI 3-Kinase 3-step HTRF™ Assay
      Product Information
      • 4X Reaction Buffer
      • PIP2 (substrate, 1mM stock)
      • DM A Reaction Buffer
      • DM B Reaction Buffer
      • DM C Reaction Buffer
      • BPIP3
      • Stop Reagent
      Detection methodHTRF
      Quality LevelMQ100
      ApplicationEverything you need to perform the PI3 Kinase 3-step HTRF assays (384 wells).
      Key Applications
      • Kinase Assay
      Biological Information
      Species Reactivity
      • Human
      • Mouse
      Analytes Available
      • PI 3-Kinase
      Entrez Gene Number
      Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
      Gene Symbol
      • PIK3CA
      • MGC142163
      • PI3K
      • MGC142161
      • p110-alpha
      Target Sub-FamilyPI3K/PI4K
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
      SIZE: 1068 amino acids; 124284 Da
      SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
      DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
      SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions12 months at -70°C
      Packaging Information
      Material Size384 wells
      Material PackageKit capacity: 384 wells
      Transport Information
      Supplemental Information

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      33-041 PI 3-Kinase 3-step HTRF™ Assay Show Pricing & Availability
      33-047 PI 3-Kinase 3-step HTRF™ Assay Show Pricing & Availability

      Alternative Format

      Catalog Number Description  
      33-016 PI 3-Kinase HTRF™ Assay; 385 wells Show Pricing & Availability

      Product Families

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      Life Science Research > Protein Detection and Quantification > Activity Assays > Kinase Assays