Millipore Sigma Vibrant Logo

71978 One Step RT-PCR Master Mix Kit

71978
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
71978-3
Retrieving availability...
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Plastic ampoule 50 rxn
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      Request Pricing
      Description
      OverviewConvenient one-enzyme, hot start master mix system for RT-PCR

      The One Step RT-PCR Master Mix Kit* allows rapid, sensitive analysis of gene expression from tissues and cells. One Step RT-PCR Master Mix Kit can replace methods for detecting and quantifying gene expression such as Northern blots, in situ hybridization, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilizes recombinant Thermus thermophilus (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimized buffer, and ultrapure deoxynucleotides. Antibody-mediated hot start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

      We recommend using either two gene-specific primers or oligo (dT) and one gene-specific 5'-primer with the kit. Although rTth adds 3' dA overhangs, it is generally not recommended for PCR product cloning because the rTth error rate is higher than standard Taq DNA Polymerase. This kit is ideal for the rapid screening of gene expression. Each kit provides sufficient reagents to perform 50 RT-PCR analyses. Positive Control RNA and Control Primers are also included.

      Features and Benefits:

      • Robust one-step, one-enzyme master mix system for easy reaction assembly
      • Eliminates the risk of cross contamination associated with two-step RT-PCR protocols
      • High-temperature (60 C) for reverse transcription enhances read-through of RNA secondary structure
      • Ideal for gene expression studies
      • Optimized buffer conditions and antibody-mediated hot start for increased sensitivity
      • Rapid enzyme activation step (30 s) avoids damage of template RNA

      Components:

      • 2 x 625 µl 2X One Step RT-PCR Master Mix
      • 1 x 200 µl 50 mM Mn(OAc)2
      • 1 x 1.1 ml RNase Free Water

      *Manufactured by Toyobo and distributed by EMD. Not available from in Japan.

      Catalogue Number71978
      Brand Family Novagen®
      References
      Product Information
      Declaration

      *Manufactured by Toyobo and distributed by EMD. Not available from in Japan.

      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications