509290 OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

509290
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Overview

Replacement Information

Key Specifications Table

Empirical FormulaCAS #
C₂₀H₄₀N₄O₁₀ · 2H₂SO₄ 108321-42-2

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                      Description
                      OverviewAminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
                      Catalogue Number509290
                      Brand Family Calbiochem®
                      SynonymsNMD Inhibitor I
                      References
                      ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
                      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
                      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
                      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
                      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
                      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
                      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
                      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
                      Jimenez, A., and Davies, J. 1980. Nature 287, 869.
                      Product Information
                      CAS number108321-42-2
                      FormWhite powder
                      Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
                      Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
                      Quality LevelMQ200
                      Applications
                      Biological Information
                      Biological activityAssay: ≥730 µg/mg
                      Purity≥90% by HPLC
                      Physicochemical Information
                      ContaminantsHeavy metals: ≤100 ppm
                      UV Absorbance (280 nm, 100 mg/ml in H₂O) ≤ 0.35
                      Dimensions
                      Materials Information
                      Toxicological Information
                      Safety Information according to GHS
                      RTECSCB9378500
                      Safety Information
                      Product Usage Statements
                      Storage and Shipping Information
                      Ship Code Ambient Temperature Only
                      Toxicity Standard Handling
                      Storage +15°C to +30°C
                      Do not freeze Ok to freeze
                      Special InstructionsSolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
                      Packaging Information
                      Transport Information
                      Supplemental Information
                      Specifications

                      Documentation

                      OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem SDS

                      Title

                      Safety Data Sheet (SDS) 

                      OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Certificates of Analysis

                      TitleLot Number
                      509290

                      References

                      Reference overview
                      Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
                      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
                      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
                      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
                      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
                      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
                      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
                      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
                      Jimenez, A., and Davies, J. 1980. Nature 287, 869.
                      Data Sheet

                      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                      Revision23-March-2020 JSW
                      SynonymsNMD Inhibitor I
                      DescriptionAminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3'-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
                      FormWhite powder
                      Recommended reaction conditionsThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 mg/ml Mammalian cells: 0.1-2.0 mg/ml A multiplying cell will be affected by the presence of G418 sooner than a resting cell. It will take at least two cell generations to achieve cell death in sensitive cell lines.
                      CAS number108321-42-2
                      RTECSCB9378500
                      Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
                      Purity≥90% by HPLC
                      ContaminantsHeavy metals: ≤100 ppm
                      UV Absorbance (280 nm, 100 mg/ml in H₂O) ≤ 0.35
                      Biological activityAssay: ≥730 µg/mg
                      Storage +15°C to +30°C
                      Do Not Freeze Ok to freeze
                      Special InstructionsSolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
                      Toxicity Standard Handling
                      ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
                      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
                      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
                      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
                      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
                      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
                      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
                      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
                      Jimenez, A., and Davies, J. 1980. Nature 287, 869.