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509290 OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

509290
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Key Specifications Table

Empirical FormulaCAS #
C₂₀H₄₀N₄O₁₀ · 2H₂SO₄ 108321-42-2

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              Description
              OverviewAminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
              Catalogue Number509290
              Brand Family Calbiochem®
              SynonymsNMD Inhibitor I
              References
              ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
              Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
              Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
              Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
              Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
              Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
              Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
              Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
              Jimenez, A., and Davies, J. 1980. Nature 287, 869.
              Product Information
              CAS number108321-42-2
              FormWhite powder
              Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
              Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
              Quality LevelMQ200
              Applications
              Biological Information
              Biological activityAssay: ≥730 µg/mg
              Purity≥90% by HPLC
              Physicochemical Information
              ContaminantsHeavy metals: ≤100 ppm
              UV Absorbance (280 nm, 100 mg/ml in H₂O) ≤ 0.35
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSCB9378500
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Standard Handling
              Storage +15°C to +30°C
              Do not freeze Ok to freeze
              Special InstructionsSolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem SDS

              Title

              Safety Data Sheet (SDS) 

              OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Certificates of Analysis

              TitleLot Number
              509290

              References

              Reference overview
              Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
              Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
              Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
              Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
              Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
              Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
              Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
              Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
              Jimenez, A., and Davies, J. 1980. Nature 287, 869.
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision23-March-2020 JSW
              SynonymsNMD Inhibitor I
              DescriptionAminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3'-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
              FormWhite powder
              Recommended reaction conditionsThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 mg/ml Mammalian cells: 0.1-2.0 mg/ml A multiplying cell will be affected by the presence of G418 sooner than a resting cell. It will take at least two cell generations to achieve cell death in sensitive cell lines.
              CAS number108321-42-2
              RTECSCB9378500
              Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
              Purity≥90% by HPLC
              ContaminantsHeavy metals: ≤100 ppm
              UV Absorbance (280 nm, 100 mg/ml in H₂O) ≤ 0.35
              Biological activityAssay: ≥730 µg/mg
              Storage +15°C to +30°C
              Do Not Freeze Ok to freeze
              Special InstructionsSolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
              Toxicity Standard Handling
              ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
              Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
              Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
              Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
              Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
              Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
              Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
              Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
              Jimenez, A., and Davies, J. 1980. Nature 287, 869.