NC010 Nerve Growth Factor 7.0s

NC010
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      SpeciesSourcePurity
      Mouse Mouse submaxillary gland (Darling & Shooter, 1984). 97% pure as determined by SDS-PAGE.
      Description
      Catalogue NumberNC010
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionNerve Growth Factor 7.0s
      OverviewMOLECULAR WT.:

      130-140 kD. 7S NGF is composed of 3 subunits; 1 beta subunit (2.5S), 2 alpha subunits and 2 gamma subunits.
      Alternate Names
      • holo-NGF
      Background InformationIn the mouse submaxillary gland the neurotrophically active NGF, beta-NGF (2.5S), is part of a larger protein complex, 7S-NGF, which has the stoichiometry alpha2betagamma2 (+ 1 or 2 zinc ions) where the alpha and gamma subunits are members of the glandular kallikrein family of serine proteases, each of about 26 000 kDa molecular weight. The alpha and gamma subunits actually inhibit beta-NGF action and must be dissociated for biological activity to be observed.



      This 7S complex has not been shown to occur in other mouse tissues, or in other species, probably because the alpha and gamma subunits, which are members of a larger glandular kallikrein family, apparently have a very restricted expression. The alpha and gamma subunits, members of the serine proteinase family, have quite similar amino acid sequences, although only the gamma subunit is catalytically active. The alpha subunit has undergone several mutations that preclude this function, including loss of the cleavage site needed for processing from the zymogen to the active form.
      References
      Product Information
      PresentationLyophilized. NC010 should be reconstituted with sterile DMEM at a concentration of

      10 μg/mL and stored at -70°C. Medium with mNGF cannot be reused due to mNGF degradation.
      Quality LevelMQ100
      Applications
      Biological Information
      Concentration1 mg/mL
      Purity97% pure as determined by SDS-PAGE.
      SourceMouse submaxillary gland (Darling & Shooter, 1984).
      Specific ActivityBIOLOGICAL ASSAY: <br /><br />The neurotrophic activity is in the range of 0.5-50 ng/mL using rat PC12 cells cultured for 7-14 days. The recommended concentration to be used in vitro for maintenance of sympathetic and immature sensory nerve cells is 50-100 ng/mL medium (Varon et al., 1967).
      Gene Symbol
      • KLK1B4
      • K1KB4
      • KLK4
      • NGFA
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Nerve Growth Factor 7.0s SDS

      Title

      Safety Data Sheet (SDS) 

      Nerve Growth Factor 7.0s Certificates of Analysis

      TitleLot Number
      7S NERVE GROWTH FACTOR (NGF)3054655
      7S NERVE GROWTH FACTOR (NGF) (High Molecular Weight Complex - 22763232276323
      7S NERVE GROWTH FACTOR (NGF) (High Molecular Weight Complex)3114942
      7S NERVE GROWTH FACTOR (NGF) (High Molecular Weight Complex)3113910
      7S NERVE GROWTH FACTOR (NGF) (High Molecular Weight Complex)2933281
      7S NERVE GROWTH FACTOR (NGF) (High Molecular Weight Complex)3123655
      7S NERVE GROWTH FACTOR (NGF) (High Molecular Weight Complex)3130502
      7S NERVE GROWTH FACTOR (NGF) (High Molecular Weight Complex)3131871
      7S NERVE GROWTH FACTOR (NGF) (High Molecular Weight Complex) - 19973721997372
      7S NERVE GROWTH FACTOR (NGF) (High Molecular Weight Complex) - 26952662695266

      References

      Reference overviewPub Med ID
      The isolation of the mouse nerve growth factor protein in a high molecular weight form.
      Varon, S, et al.
      Biochemistry, 6: 2202-9 (1967)  1967

      6049455 6049455

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors