NC011 Nerve Growth Factor 2.5s Protein, Mouse submaxillary glands

NC011
50 µg  2x25 μg
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      Overview

      Replacement Information

      Key Specifications Table

      SpeciesSourcePurity
      Mouse Mouse submaxillary gland. Greater than 90% determined by SDS-PAGE electrophoresis.
      Description
      Catalogue NumberNC011
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionNerve Growth Factor 2.5s Protein, Mouse submaxillary glands
      OverviewMOLECULAR WT.:

      13,000 reduced
      Alternate Names
      • beta-NGF
      Background InformationMurine 2.5S Nerve Growth Factor is a 26kDa protein composed of two identical 118 amino acid chains under native conditions.. The 2.5S form of murine Nerve Growth Factor originates from the dissociation and proteolytic cleavage of the beta subunit of 7S forms of this neurotrophic factor and is also referred to as beta-NGF. This form mediates phosphorylation of specific intracellular proteins and is solely responsible for the biological activity of both the 2.5S and 7S forms of this neurotrophic factor. Murine 2.5S Nerve Growth Factor is expressed in sympathetic and sensory-innervated peripheral tissues such as the vas deferens, heart, iris, skin, splenic capsule, sciatic nerve and submaxillary gland. At the cellular level, mNGF expression has been demonstrated in lymphocytes, smooth muscle cells, epithelial cells, astrocytes, fibroblasts and Schwann cells. There is a strong correlation between the levels of mNGF mRNA in peripheral tissues and the density of sympathetic innervation. Peripheral neurons that respond to mNGF include adrenergic sympathetic neurons and primary sensory neurons. Cellular responses to this neurotrophic factor include differentiation, neurotic extension and production of catecholamine and neuropeptide-synthesizing enzymes. Murine Nerve Growth Factor binds the "low affinity" NGF receptor (p75NTR), as do all neurotrophic factors, and it mediates, via p75NTR signal transduction, the functional response of Schwann cells. mNGF also activates signal transduction by the dimerization and autophosphorylation of the TrkA receptor (also known as Trk and p140trk).
      References
      Product Information
      PresentationLyophilized. Reconstitute to a concentration of 10 μg/mL with sterile DMEM. Medium with mNGF cannot be reused due to mNGF degradation.
      Quality LevelMQ100
      Applications
      ApplicationThe murine 2.5S Nerve Growth Factor (or NGF protein) is a 26kDa protein composed of two identical 118 amino acid chains under native conditions.
      Biological Information
      Concentration1 mg/mL
      PurityGreater than 90% determined by SDS-PAGE electrophoresis.
      SourceMouse submaxillary gland.
      Specific ActivityBIOLOGICAL ASSAY: <br /><br />This lot was bioassayed for neurotrophic activity using rat PC12 cell cultures over a period of 7-14 days. The activity is in the range of 0.5-50 ng/mL. The recommended concentration to be used in vitro for maintenance of sympathetic and sensory nerve cultures is 50 ng/mL of medium (Bradshaw, 1978).
      Gene Symbol
      • NGF
      • NGFB
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain frozen at -70°C in undiluted aliquots for 4-6 weeks. Avoid repeated freeze-thaw cycles as it may result in a loss of activity.
      Packaging Information
      Material Size50 µg
      Material Package2x25 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Nerve Growth Factor 2.5s Protein, Mouse submaxillary glands SDS

      Title

      Safety Data Sheet (SDS) 

      Nerve Growth Factor 2.5s Protein, Mouse submaxillary glands Certificates of Analysis

      TitleLot Number
      NERVE GROWTH FACTOR 2.5S (Tissue Culture Grade) - 23792862379286
      NERVE GROWTH FACTOR 2.5S (Tissue Culture Grade)2928412
      NERVE GROWTH FACTOR 2.5S (Tissue Culture Grade)2902309
      NERVE GROWTH FACTOR 2.5S (Tissue Culture Grade)3128169
      NERVE GROWTH FACTOR 2.5S (Tissue Culture Grade)3015912
      NERVE GROWTH FACTOR 2.5S (Tissue Culture Grade)3073791
      NERVE GROWTH FACTOR 2.5S (Tissue Culture Grade)2891315
      NERVE GROWTH FACTOR 2.5S (Tissue Culture Grade)2869296
      NERVE GROWTH FACTOR 2.5S (Tissue Culture Grade) - 19994451999445
      NERVE GROWTH FACTOR 2.5S (Tissue Culture Grade) - 31504243150424

      References

      Reference overviewPub Med ID
      Recording of mechanosensitive currents using piezoelectrically driven mechanostimulator.
      Jizhe Hao,Patrick Delmas
      Nature protocols  6  2011

      Show Abstract
      21720312 21720312
      Nerve growth factor.
      Bradshaw, R A
      Annu. Rev. Biochem., 47: 191-216 (1978)  1978

      79332 79332

      Data Sheet

      Title
      NERVE GROWTH FACTOR 2.5S

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors