492027 NRG1 Isoform SMDF, Human, Recombinant

492027
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Overview

Replacement Information

Key Specifications Table

Purity
>95% by SDS-PAGE

Pricing & Availability

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492027-25UG
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      Glass bottle 25 μg
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      Description
      OverviewRecombinant, human neuregulin 1 (NRG1), isoform SMDF (sensory and motor neuron-derived factor), consisting of amino acids 1-263, 117-296, and 162-296, each fused at the C-terminus to a His•Tag® sequence and expressed in S. frugiperda Sf21 cells using a baculovirus expression system. The neuregulin (also known as heregulin) family of cytokines is comprised of multiple secreted or membrane-bound isorforms that are produced from the single heregulin gene by alternative splicing and/or usage of different promoters. NRG1 isoform SMDF is a type III isoform with a C-terminal EGF-like domain that interacts with the erbB family of tyrosine kinase receptors. NRG1 isoform SMDF expression has been found to be restricted to the nervous system and is believed to play an important role in the development of the nervous system.
      Catalogue Number492027
      Brand Family Calbiochem®
      SynonymsHeregulin-1
      References
      ReferencesMeyer, D. et al. 1997. Development 124, 3575.
      Yarden, Y. and D. Wen 1994. in Guidebook to Cytokinesand Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146.
      Karey, K.P., et al. 1988. Cancer Res. 48, 4083.
      Product Information
      FormLyophilized
      FormulationLyophilized from 0.2 µm filtered PBS, BSA.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 1-5 ng/ml as measured in a serum-free proliferation assay using MCF-7 human breast adenocarcinoma cells.
      Purity>95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1.0 EU/µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstituion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      NRG1 Isoform SMDF, Human, Recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      NRG1 Isoform SMDF, Human, Recombinant Certificates of Analysis

      TitleLot Number
      492027

      References

      Reference overview
      Meyer, D. et al. 1997. Development 124, 3575.
      Yarden, Y. and D. Wen 1994. in Guidebook to Cytokinesand Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146.
      Karey, K.P., et al. 1988. Cancer Res. 48, 4083.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-June-2011 RFH
      SynonymsHeregulin-1
      DescriptionRecombinant, human neuregulin 1 (NRG1), isoform SMDF (sensory and motor neuron-derived factor), consisting of amino acids 1-263, 117-296, and 162-296, each fused at the C-terminus to a His•Tag® sequence and expressed in S. frugiperda Sf21 cells using a baculovirus expression system. The neuregulin (also known as heregulin) family of cytokines is comprised of multiple secreted or membrane-bound isorforms that are produced from the single heregulin gene by alternative splicing and/or usage of different promoters. NRG1 isoform SMDF is a type III isoform with a C-terminal EGF-like domain that interacts with the erbB family of tyrosine kinase receptors. NRG1 isoform SMDF expression has been found to be restricted to the nervous system and is believed to play an important role in the development of the nervous system.
      FormLyophilized
      FormulationLyophilized from 0.2 µm filtered PBS, BSA.
      Purity>95% by SDS-PAGE
      ContaminantsEndotoxin: ≤1.0 EU/µg protein
      Biological activityED₅₀ = 1-5 ng/ml as measured in a serum-free proliferation assay using MCF-7 human breast adenocarcinoma cells.
      SolubilityReconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstituion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesMeyer, D. et al. 1997. Development 124, 3575.
      Yarden, Y. and D. Wen 1994. in Guidebook to Cytokinesand Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146.
      Karey, K.P., et al. 1988. Cancer Res. 48, 4083.