Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

532759 NOX1 Inhibitor, NoxA1ds Set - Calbiochem

A cell-permeable peptide that acts as a highly potent,isoform specific inhibitor of NADPH oxidase 1 (IC₅₀ = 19 nM). Binds to NOX1 and disrupts its interaction with its activator subunit (NOXA1).

NOX1 Inhibitor, NoxA1ds Set - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: NADPH Oxidase 1 Inhibitor, NoxA1ds, NADPH-oxidase inhibitor, NoxA1ds

Primary Target: NOX1
532759
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₅₀H₈₈N₁₄O₁₅

Products

Catalog NumberPackaging Qty/Pack
5.32759.0001 Glass bottle 1 set
Description
OverviewA cell permeable peptide derived from the activation domain of NOXA1, with Y199A substitution that acts as a highly potent and isoform specific inhibitor of NADPH oxidase 1 (NOX1; IC50 = 19 nM). Acts by binding to NOX1 and disrupts its interaction with its activator subunit (NOXA1). Shown to block NOX1-derived production of superoxide ( O2.- ) in a reconstituted NOX1 cell-free system, but does not affect NOX2, NOX4, NOX5 or xanthine oxidase derived superoxide and has no scavenging effects on either superoxide or hydrogen peroxide. Also suppresses superoxide production in NOX1 expressing HT-29 colon carcinoma cells (IC50 = 100 nM) and in hypoxia-induced human pulmonary artery endothelial cells (HPAEC), but does not affect O2.- production in peritoneal macropages derived from NOX1-null mice. A scrambled peptide is also included as a negative control.

Please note that the molecular weight for this compound is batch-specific due to variable water content.
Catalogue Number532759
Brand Family Calbiochem®
SynonymsNADPH Oxidase 1 Inhibitor, NoxA1ds, NADPH-oxidase inhibitor, NoxA1ds
References
ReferencesRanayhossaini, D.J., et al. 2013. J. Bio. Chem. 288, 36437.
Product Information
FormWhite solid
Formulation1 set contains 5 mg NOX1 Inhibitor, NoxA1ds (Cat. No. 5.32760.0001) and 5 mg NOX1 Inhibitor, NoxA1ds Negative Control (Cat. No. 5.32761.0001).
Hill FormulaC₅₀H₈₈N₁₄O₁₅
Chemical formulaC₅₀H₈₈N₁₄O₁₅
Hygroscopic Hygroscopic
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetNOX1
Primary Target IC<sub>50</sub>19 nM
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Peptide SequenceNOX1 Inhibitor, NoxA1ds: NH3-EPVDALGKAKV-CONH2
NOX1 Inhibitor, NoxA1ds Negative Control: NH2-LVKGPDAEKVA-CONH2
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
5.32759.0001 04055977281903

Documentation

NOX1 Inhibitor, NoxA1ds Set - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Ranayhossaini, D.J., et al. 2013. J. Bio. Chem. 288, 36437.

Brochure

Title
Neuroscience Solutions for productive research
Pathways and Biomarkers of Oxidative Stress

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-September-2020 JSW
SynonymsNADPH Oxidase 1 Inhibitor, NoxA1ds, NADPH-oxidase inhibitor, NoxA1ds
FormWhite solid
Formulation1 set contains 5 mg NOX1 Inhibitor, NoxA1ds (Cat. No. 5.32760.0001) and 5 mg NOX1 Inhibitor, NoxA1ds Negative Control (Cat. No. 5.32761.0001).
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₅₀H₈₈N₁₄O₁₅
Peptide SequenceNOX1 Inhibitor, NoxA1ds: NH3-EPVDALGKAKV-CONH2
NOX1 Inhibitor, NoxA1ds Negative Control: NH2-LVKGPDAEKVA-CONH2
Purity≥98% by HPLC
SolubilityH₂0 (50 mg/ml), DMSO (50 mg/ml), or Methanol (50 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesRanayhossaini, D.J., et al. 2013. J. Bio. Chem. 288, 36437.