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480012 NDSB Set - Calbiochem

480012
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480012-1SET
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      Description
      OverviewContains the following non-detergent sulfobetaines: 5 g of NDSB-195 (Cat. No. 480001), 25 g of NDSB-201 (Cat. No. 480005), and 5 g of NDSB-256 (Cat. No. 480010). Supplied with a data sheet.
      Catalogue Number480012
      Brand Family Calbiochem®
      References
      ReferencesGoldberg, M.E., et al. 1996. Folding and Design 1, 21.
      Goldberg, M.E., et al. 1995. Folding & Design 1, 21.
      Vuillard, L., et al. 1995. Biochem. J. 305, 337.
      Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.
      Vuillard, L., et al. 1994. FEBS Lett. 353, 294.
      Product Information
      FormSolid
      Hygroscopic Hygroscopic
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage +15°C to +30°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsStore lyophilized products at room temperature. Following reconstitution, store in the refrigerator (4°C) or at room temperature (20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      NDSB Set - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      NDSB Set - Calbiochem Certificates of Analysis

      TitleLot Number
      480012

      References

      Reference overview
      Goldberg, M.E., et al. 1996. Folding and Design 1, 21.
      Goldberg, M.E., et al. 1995. Folding & Design 1, 21.
      Vuillard, L., et al. 1995. Biochem. J. 305, 337.
      Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.
      Vuillard, L., et al. 1994. FEBS Lett. 353, 294.

      Brochure

      Title
      Protein Purification and Detection Tools
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-August-2008 RFH
      DescriptionNon-detergent sulfobetaines (NDSBs) are zwitterionic compounds. Like zwittergents (zwitterionic detergents), NDSBs carry the sulfobetaine hydrophilic head group. However, unlike zwittergents, the hydrophobic group in NDSBs is too short for micellar formation even at concentrations as high as 1M. Hence, NDSBs do not behave like detergents. Because of their zwitterionic nature, NDSBs were first employed to neutralize electrostatic interactions without increasing the conductivity in native isoelectrofocusing gels. Recently they have found utility in various other applications including isolation of membrane proteins, purification of nuclear proteins and halophilic proteins, renaturation of chemically and thermally denatured proteins and protein crystallization. They are potentially useful in solubilization and renaturation of fusion proteins from inclusion bodies. It is hypothesized that the hydrophobic group, although short, interacts with the hydrophobic regions of the protein preventing aggregation during renaturation. Also, presumably, the contribution from the short hydrophobic groups combined with charge neutralization ability of the sulfobetaine group results in higher yields of membrane proteins.

      NDSBs do not interfere with enzymatic assays based on chromogenic substrates bearing nitrophenyl groups and do not inhibit the activities of β-galactosidase and alkaline phosphatase, the two enzymes tested thus far. NDSB-195 has the added advantage in that it does not absorb at 280 nm, making it compatible with protein purification procedures in which the protein concentrations are determined by absorbance measurements at 280 nm. Supplied with a data sheet.
      FormSolid
      SolubilityH₂O (50 mg/ml)
      Storage +15°C to +30°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsStore lyophilized products at room temperature. Following reconstitution, store in the refrigerator (4°C) or at room temperature (20°C).
      Toxicity Irritant
      ReferencesGoldberg, M.E., et al. 1996. Folding and Design 1, 21.
      Goldberg, M.E., et al. 1995. Folding & Design 1, 21.
      Vuillard, L., et al. 1995. Biochem. J. 305, 337.
      Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.
      Vuillard, L., et al. 1994. FEBS Lett. 353, 294.

      Related Products & Applications

      Related Products

      Catalog Number Description  
      480001 NDSB-195 - CAS 160255-06-1 - Calbiochem Show Pricing & Availability
      480005 NDSB-201 - CAS 15471-17-7 - Calbiochem Show Pricing & Availability
      480010 NDSB-256 - CAS 81239-45-4 - Calbiochem Show Pricing & Availability

      Related Products

      Catalog Number Description  
      480001 NDSB-195 - CAS 160255-06-1 - Calbiochem Show Pricing & Availability
      480005 NDSB-201 - CAS 15471-17-7 - Calbiochem Show Pricing & Availability
      480010 NDSB-256 - CAS 81239-45-4 - Calbiochem Show Pricing & Availability

      Categories

      Life Science Research > Inhibitors and Biochemicals > Biochemicals > Detergents > Non-Detergent Sulfobetaines