533998 Mitotic Tracker Probe, CDy6 - Calbiochem

533998
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₃₄H₄₇BF₂N₄O₂

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      Glass bottle 5 mg
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      Description
      OverviewA cell-permeable, photostable, bodipy-derived fluorescent probe that can be used for real-time imaging of lysosomal dynamics in proliferating cells undergoing mitosis. Undergoes rapid re-localization from cytoplasm to nucleus during cell division, but does not interfere with cell cycle. Displays a sharp increase in intensity during mitosis and its fluorescence displays high sensitivity to acidic environment (pH 2-4). Can also be used for time-dependent whole-body in vivo imaging of tumor xenografts (CDy6 stained HeLa cells, s.c.) in BALB/c nude mice up to 21 days. Exhibits greater brightness, higher photostability, and significantly reduced toxicity when compared to LysoTracker Red, LysoTracker Green, LysoSensor Green and HOE33342. Abs max: 570 nm; Em max: 585 with a quantum yield of 0.89.

      Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
      Catalogue Number533998
      Brand Family Calbiochem®
      Synonyms(E)-7-(3,5-di-tert-Butyl-2-methoxystyryl)-3-(3-(2-(dimethylamino)ethylamino)-3-oxopropyl)-5,5-difluoro-9-methyl-5H-dipyrrolo[1,2-c:1ʹ,2ʹ-f][1,3,2]diazaborinin-4-ium-5-uide, Lysosomal Tracker Probe, CDy6
      References
      ReferencesJeong, Y.M., et al. 2015. Chem. Biol. 22, 299.
      Product Information
      FormDark purple powder
      Hill FormulaC₃₄H₄₇BF₂N₄O₂
      Chemical formulaC₃₄H₄₇BF₂N₄O₂
      ReversibleY
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Targetcytoplasm
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C). Store in a dark vial, protected from light.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Mitotic Tracker Probe, CDy6 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Jeong, Y.M., et al. 2015. Chem. Biol. 22, 299.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-December-2016 JSW
      Synonyms(E)-7-(3,5-di-tert-Butyl-2-methoxystyryl)-3-(3-(2-(dimethylamino)ethylamino)-3-oxopropyl)-5,5-difluoro-9-methyl-5H-dipyrrolo[1,2-c:1ʹ,2ʹ-f][1,3,2]diazaborinin-4-ium-5-uide, Lysosomal Tracker Probe, CDy6
      DescriptionA cell-permeable, photostable, bodipy-derived fluorescent probe that can be used for real-time imaging of lysosomal dynamics in proliferating cells undergoing mitosis. Undergoes rapid re-localization from cytoplasm to nucleus during cell division, but does not interfere with cell cycle. Displays a sharp increase in intensity during mitosis and its fluorescence displays high sensitivity to acidic environment (pH 2-4). Can also be used for time-dependent whole-body in vivo imaging of tumor xenografts (CDy6 stained HeLa cells, s.c.) in BALB/c nude mice up to 21 days. Exhibits greater brightness, higher photostability, and significantly reduced toxicity when compared to LysoTracker Red, LysoTracker Green, LysoSensor Green and HOE33342. Abs max: 570 nm; Em max: 585 with a quantum yield of 0.89.
      FormDark purple powder
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₃₄H₄₇BF₂N₄O₂
      Purity≥98% by HPLC
      SolubilityDMSO (10 mg/ml). Use only fresh, anhydrous DMSO for reconstitution.
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C). Store in a dark vial, protected from light.
      Toxicity Standard Handling
      ReferencesJeong, Y.M., et al. 2015. Chem. Biol. 22, 299.