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Clinical Chemistry and Laboratory Medicine 37/1 (37-45) 1999 1999
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|How can I dispose of a used Minicon device?||You can dispose of a Minicon by autoclaving it or incinerating it.|
|Which Minicon do I use?||The Minicon unit you chose should be based on the fluid being processed. See below.
Urine - B15 or Miniplus
Cerebrospinal Fluid - B15 or CS15
Dilute body fluid - A25
|What is the molecular weight cut-off of the Minicon unit?||The molecular weight cut-off varies with the device. The ratings for the Minicon devices are listed below.
|Will the Minicon unit concentrate to dryness?||At about 100x, the membrane has been treated to retard further concentration and accidental reduction to dryness. However, Spinal fluid and other very dilute specimens may occasionally concentrate beyond 100x. To assure recovery of valuable sample, check concentration level periodically and remove concentrate once it has reached 100x. If sample appears to go to dryness, pipette up to 0.5ml of saline or buffer into the sample chamber. Mix concentrate by drawing liquid in and out of a pipette several times before removal from chamber.|
|Are the Minicon test results quantitative?||No. There is some nonspecific binding of solute on the membrane as well as non-recoverable film loss due to wetting of the chamber that is not recoverable.|
|Can the Minicon cell be refilled? How many times?||Yes, the units can be refilled. See the following chart for specific devices. Minicon - B15 2 times Minicon - CS15 2 times Minicon - A25 3 times Miniplus 2 times|
|How many tests are there in each Minicon?||The number of tests vary with the device. See below for details.
|How much sample does each Minicon test cell hold?||The maximum cell capacities are as follows:
|Can I use Minicon after its expiration date?||It is not recommended. The flow rates will slow down after the expiration date.|
|Can Minicon be reused?||Yes. The unused chambers can be used if a bacteriastat such as sodium azide is added to any used chamber and the Minicon is stored refrigerated.|