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Millicell® Cell Culture Inserts

Optimized cell growth, attachment and differentiation

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Overview

Specifications

Ordering Information

Millicell Standing InsertsClear Sorting & Filtering Show Filter
Catalog NumberMediaChemistryPore SizeFiltration AreaDevice ConfigurationPack Size
PICM0RG50Biopore® Hydrophilic Polytetrafluoroethylene (PTFE) 0.4 µm 4.2 cm² 6-well plate 50
PIHA01250MF-Millipore Mixed Cellulose Esters (MCE) 0.45 µm 0.6 cm² 24-well plate 50
PIHA03050MF-Millipore Mixed Cellulose Esters (MCE) 0.45 µm 4.2 cm² 6-well plate 50
PICM01250Biopore® Hydrophilic Polytetrafluoroethylene (PTFE) 0.4 µm 0.6 cm² 24-well plate 50
PICM03050Biopore® Hydrophilic Polytetrafluoroethylene (PTFE) 0.4 µm 4.2 cm² 6-well plate 50
PIHP01250Isopore® Polycarbonate (PC) 0.4 µm 0.6 cm² 24-well plate 50
PIHP03050Isopore® Polycarbonate (PC) 0.4 µm 4.2 cm² 6-well plate 50
PITP01250Isopore® Polycarbonate (PC) 3.0 µm 0.6 cm² 24-well plate 50
PIXP01250Isopore® Polycarbonate (PC) 12.0 µm 0.6 cm² 24-well plate 50
PI8P01250Isopore® Polycarbonate (PC) 8.0 µm 0.6 cm² 24-well plate 50

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Millicell Hanging InsertsClear Sorting & Filtering Show Filter
Catalog NumberChemistryPore SizeFiltration AreaDevice ConfigurationPack Size
PTEP24H48Polyethylene Terephthalate (PET) 8.0 µm 0.3 cm² 24-well plate 48/box
PTHT24H48Polyethylene Terephthalate (PET) 0.4 µm 0.3 cm² 24-well plate 48/box
PTMP24H48Polyethylene Terephthalate (PET) 5.0 µm 0.3 cm² 24-well plate 48
PTRP24H48Polyethylene Terephthalate (PET) 1.0 µm 0.3 cm² 24-well plate 48
PTSP24H48Polyethylene Terephthalate (PET) 3.0 µm 0.3 cm² 24-well plate 48
PCHT24H48Polyethylene Terephthalate (PET) 0.4 µm 0.3 cm² 24-well plate 48
PLRP24H48Polyethylene Terephthalate (PET) 1.0 µm 0.3 cm² 24-well plate 48
PCSP24H48Polyethylene Terephthalate (PET) 3.0 µm 0.3 cm² 24-well plate 48
PCHT06H48Polyethylene Terephthalate (PET) 0.4 µm 4.5 cm² 6-well plate 48
PCHT12H48Polyethylene Terephthalate (PET) 0.4 µm 1.1 cm² 12-well plate 48
PCSP06H48Polyethylene Terephthalate (PET) 3.0 µm 4.5 cm² 6-well plate 48
PCSP12H48Polyethylene Terephthalate (PET) 3.0 µm 1.1 cm² 12-well plate 48
PCEP06H48Polyethylene Terephthalate (PET) 8.0 µm 4.5 cm² 6-well plate 48
PCEP12H48Polyethylene Terephthalate (PET) 8.0 µm 1.1 cm² 12-well plate 48
PCEP24H48Polyethylene Terephthalate (PET) 8.0 µm 0.3 cm² 24-well plate 48
PLRP06H48Polyethylene Terephthalate (PET) 1.0 µm 4.5 cm² 6-well plate 48
PLRP12H48Polyethylene Terephthalate (PET) 1.0 µm 1.1 cm² 12-well plate 48

* For attachment-dependent cells, this membrane needs to be coated with an extra cellular matrix.

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Documentation

References

Reference overviewApplication
Propagation of human embryonic stem cells in a microporous membrane-based indirect co-culture system
Kelsey Albert, Steven Sheridan, Louise Laurent, Igor Ulitsky, Ron Shamir, Jeanne Loring, & Raj R. Rao
Biochemical and Biophysical Research Communications  2010

Astrocyte growth effects of vascular endothelial growth factor (VEGF) application to perinatal neocortical explants: Receptor mediation and signal transduction pathways
Nina Mani, Alfia Khaibullina, Janette Krum and Jeffrey Rosenstein
Experimental Neurology 192 (2005); 394-406  2005

Cell Culture
Subcellular localisation of recombinant a and g-synuclein
Christian Specht, Cezar Tigaret, George Rast, Agnea Thalhammer, York Rudhard and Ralf Schoepfer
Mol. Cell. Neurosci., 28 (2005); 326-334  2005

Cell Culture
Establishment of the organotypic model of amyotrophic lateral sclerosis from the SD rats' spinal cord
Diao ZY, et. al,Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese.
Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese.  2005

Cell Culture
Neural stem cells protect against glutamate-induced excitotoxicitiy and promote survival of injured motor neurons through the secretion of neurotrophic factors
Jeronia Llado, Christine Haenggeli, Nicholas Maragakis, Evan Snyder and Jeffrey Rothstein
Mol. Cell. Neurosci. , 27 (2004); 322-331  2004

Cell Culture
Development of an in vitro blood-brain barrier model-cytotoxicity of mercury and aluminum.
Toimela, T et. al.,Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.
Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.  2004

Morphological differentiation of bone marrow stromal cells into neuron-like cells after co-culture with hippocampal slice
Abouelfetouh, Ayman, et al
Brain Research (2004), Volume 1029, Issue 1 pp 114-119  2004

Cell Culture
Effect of sodium bicarbonate on extracellular pH, matrix accumulation, and morphology of cultured articular chondrocytes.
Waldman SD, Couto DC, Omelon SJ, Kandel RA
Tissue Engineering (2004), Nov-Dec;10(11-12):1633-40  2004

FGF-10 plays an essential role in the growth of the fetal prostate.
Annemarie A. Donjacour, Axel A. Thomson and Gerald R. Cunha
Developmental Biology 261 (1): 39-54  2003

Cell Culture
Changes in lymphokine receptor expression and fatty acid composition of phospholipids and triacylglycerols in rat adipocytes associated with lymph nodes following a transient immune challenge.
J. D. Priddle, C. A. Mattacks, D. A. Sadler, H. A. MacQueen and C. M. Pond
Cell Biology International 27 (1): 23-29  2003

Cell Differentiation

FAQ

QuestionAnswer
Why do the electrodes need to be replaced every six months?In general, electrodes have a 6 month lifetime if they are used continuously. The electrodes have a silver-silver chloride pellete that is depleted everytime it is used. It is the silver silver chloride pellete that creates the potential.
What voltage value can I expect for my cell type?We DO NOT recommend using this function on the instrument. The Millicell-ERS will make voltage readings on only a few kinds of high-voltage generating cells (for example, it will not work on MDCK cells). We do have information on epithelial cells which typically have voltage values of 0-30mV.
What resistance value can I expect for my cell types?Some resistance values have been published for various cell types. Resistance measurements will also depend on the type of cell culture (i.e. cell line, tissue or native cells). In general, the resistance range for epithelial cells is 10-20,000 ohms/cm2. An example of an established epithelial cell line with well defined electrical resistance is MDCK cells. MDCK cells displaying tight junctions show resistance of 5000 ohms/cm2.
What is the porosity of the Millicell Culture Plate Inserts?The porosity of the Millicell Insert membranes are as follows: HATF - approx 80% CM - approx 80% PC and PCF - approx 10%
What is the length of the legs on the Millicell Insert unit?The legs on the Millicell unit are approximately 1-2mm.
What is the thickness of the Millicell-PC and -PCF membranes?The thickness of the PC and PCF membranes are 10 um.
What is the thickness of the Millicell-CM membrane?The thickness of the Millicell-CM memnbrane is approximately 50 um.
What is the overall height of the Millicell-CM Organotypic insert?The overall wall height including the feet is 5 mm.
What Volume of media should be used on the inside and outside of the 12 mm and 30mm Millicell cell culture insert devices?Inside and outside liquid heights are critical and must be adjusted until equal. The suggested volumes below can vary due to plate well volume variations allowed by different cell culture plate manufacturers.

12 mm inserts:
Inside: 0.4 mL
Outside: 0.6 mL
Which ECM coating do we recommend for endothelial cells?We recommend the Type 1 Rat Tail Collagen for most endothelial cells. It is recommended to be done with ethanol wetting and is a very cost efficient way to do ECM and seems to be well tolerated by most endothelial cells.