SCM040 Sigma-AldrichMilliTrace Rat Neural Stem Cell Expansion Medium

MilliTrace Rat Neural Stem Cell Expansion Medium is provided as a multi-component system that has been optimized for use with the MilliTrace line of GFP reporter rat neural stem cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
Technical Information
Data Sheet

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Overview

Replacement Information

Key Specifications Table

Key Applications
Cult

Description
Catalogue Number	SCM040
Trade Name	Chemicon
Description	MilliTrace Rat Neural Stem Cell Expansion Medium
Overview	MilliTrace Rat Neural Stem Cell Expansion Medium is provided as a multi-component system that has been optimized for use with the MilliTrace line of GFP reporter rat neural stem cells. The Neural Stem Cell Basal Medium is a defined serum-free medium that helps support the growth and in vitro differentiation of neural stem cells derived from rodents. Basic Fibroblast Growth Factor (FGF-2, bFGF) is provided separately, which when added to the Neural Stem Cell Basal Medium (SCM003) allows for the growth and proliferation of rat neural stem cells. Withdrawal of FGF-2 from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of rat neural stem cells. Puromycin Solution is provided separately to help maintain the expression of the GFP labeled transgene.
Materials Required but Not Delivered	· MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit (Millipore Catalog No. SCR080) · Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019) · Poly-L-ornithine (Sigma Catalog No. P3655) · Laminin (Millipore Catalog No. CC095) · Accutase™ (Millipore Catalog No. SCR005)

References

Product Information
Components	Neural Stem Cell Basal Medium, 500 mL Basic FGF, 10 µg Puromycin Solution, 100 µL

Applications
Application	MilliTrace Rat Neural Stem Cell Expansion Medium is provided as a multi-component system that has been optimized for use with the MilliTrace line of GFP reporter rat neural stem cells.
Key Applications	Stem Cell Culture

Biological Information
Media Form	Liquid
Stem Cell Type	Neural Stem Cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	· Neural Stem Cell Basal Medium (Part No. SCM003) should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month. · Basic FGF (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity. · 5 mg/mL Puromycin Solution, 100 µL (Part No. CS201361) should be stored in working aliquots at -20°C for up to two years.

Storage and Shipping Information

Storage Conditions

· Neural Stem Cell Basal Medium (Part No. SCM003) should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.
· Basic FGF (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
· 5 mg/mL Puromycin Solution, 100 µL (Part No. CS201361) should be stored in working aliquots at -20°C for up to two years.

Packaging Information
Material Size	1 kit

Transport Information

Supplemental Information

Specifications

Documentation

MilliTrace Rat Neural Stem Cell Expansion Medium SDS

Title
Safety Data Sheet (SDS)

MilliTrace Rat Neural Stem Cell Expansion Medium Certificates of Analysis

Title	Lot Number
MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - 2135108	2135108
MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - 2145944	2145944
MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - 1978029	1978029
MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - 2193299	2193299
MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - 2196014	2196014
MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - 2243347	2243347
MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - NG1865584	NG1865584
MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - NG1885812	NG1885812
MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - NG1910734	NG1910734
MILLITRACE RAT NEURAL STEM CELL EXPANSION MEDIUM - 2492798	2492798

Technical Info

Title
MilliTrace Constitutive GFP Reporter Neural Stem Cell Lines

Data Sheet

Title
MILLITRACE™ RAT NEURAL STEM CELL EXPANSION MEDIUM

Related Products & Applications

Accessories

Catalog Number

Description

SCR080

MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit

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