SCR018 Mesenchymal Stem Cell Characterization Kit

SCR018
1 kit  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      ICC, Cult
      Description
      Catalogue NumberSCR018
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionMesenchymal Stem Cell Characterization Kit
      OverviewMillipore's Mesenchymal Stem Cell Characterization Kit (Catalog Number SCR018) contains a panel of positive and negative selection markers for the characterization of the mesenchymal stem cell population in rat. Positive cell markers include antibodies directed against cell-surface molecules (integrin beta1 and CD54) (Pittenger, M. F., and Marshak, D. R., & Pittenger, M.F., et al.) present on mesenchymal stem cells along with two extracellular matrix molecules (fibronectin and type I collagen) that are synthesized by cultured mesenchymal stem cells (Prockop, D. J.). Along with the positive selection markers, two specific hematopoietic cell surface markers, (CD14 - present on leukocytes and CD45 - present on monocytes and macrophages) are provided whose expressions should not be present on mesenchymal stem cells (Pittenger, M. F., and Marshak, D. R., & Alhadlaq, A., and Mao J. J, & Pittenger, M.F.,et al.). Mouse and rabbit immunoglobulins for the assessment of background staining are also included.

      For Research Use Only; not for use in diagnostic procedure.
      Materials Required but Not Delivered1. Mesenchymal stem cells and culture reagents (Cat. No. SCR027).
      2. Mesenchymal Stem Cell Expansion Media (Cat. No. SCM015)
      3. Chamber slides
      4. Glass coverslips
      5. Phosphate-Buffered Saline (1X PBS)
      6. Fixative (e.g. freshly made 4% Paraformaldehyde in 1X PBS, pH 7.4)
      7. Blocking Solution (5% normal donkey serum, with 0.3% Triton X-100 in 1X PBS filtered through a 0.45µm filter)
      8. Non-Permeable Blocking Solution (5% normal donkey serum in 1X PBS, filtered through a 0.45µm filter)
      9. Fluorescent-labeled secondary antibodies. Donkey anti-mouse IgG, Cy3 conjugated (Cat. No. AP192C) and donkey anti-rabbit IgG, Cy3 conjugated (Cat. No. AP182C) are recommended
      10. Antibody dilution buffer (1X PBS without blocking or detergents).
      11. 4'-6-Diamidino-2-phenylindole (DAPI) / PBS solution (200ng/mL in PBS). Note keep solution out of light.
      12. Anti-fading mounting solution (Cat. No. 5013).
      13. Fluorescent Microscope
      References
      Product Information
      Components
      • Rabbit anti-Integrin β1 (Catalog No. AB1952-20): Known species reactivity: Hu, Ms, Rt, other species not tested. One vial containing 20 µL polyclonal antibody.
      • Mouse anti-CD54 (ICAM-1)(Catalog No. MAB2130, clone W-CAM-1): Known species reactivity: Hu., other species not tested. One vial containing 100 µL monoclonal antibody.
      • Rabbit anti-Collagen Type I (Catalog No. 2003605): Known species reactivity: Rt, other species not tested. One vial containing 20 µL polyclonal antibody.
      • Rabbit anti-Fibronectin (Catalog No. 2003606): Known species reactivity: Rt, other species not tested. One vial containing 10 µg polyclonal antibody.
      • Mouse anti-CD45 (Catalog No. 2003607, clone OX-1): Known species reactivity: Rt, other species not tested. One vial containing 100 µL monoclonal antibody.
      • Mouse anti CD14 (Catalog No. 2003608, clone UCHM-1): Known species reactivity: Hu, Mky, other species not tested. One vial containing 10 µL monoclonal antibody.
      • Mouse IgG (Catalog No. PP54-100UG): One vial containing 100 µg purified mouse IgG control antibody.
      • Rabbit IgG (Catalog No. PP64-100UG): One vial containing 100 µg purified rabbit IgG antibody.
      Applications
      ApplicationThis Mesenchymal Stem Cell Characterization Kit contains a panel of positive & negative selection markers for the characterization of the mesenchymal stem cell population in rat.
      Key Applications
      • Immunocytochemistry
      • Stem Cell Culture
      Biological Information
      Stem Cell Type
      • Pancreatic Stem Cells
      • Mesenchymal Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWhen stored at -20°C, components are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References

      Reference overviewPub Med ID
      Multipotent properties of myofibroblast cells derived from human placenta.
      Strakova, Z; Livak, M; Krezalek, M; Ihnatovych, I
      Cell and tissue research  332  479-88  2008

      Show Abstract Full Text Article
      18401596 18401596
      Marrow stromal cells as stem cells for nonhematopoietic tissues.
      Prockop, D J
      Science, 276: 71-4 (1997)  1997

      Show Abstract
      9082988 9082988

      Technical Info

      Title
      Mesenchymal Stem Cell Products

      User Guides

      Title
      Mesenchymal Stem Cell Characterization Kit (Rat)