17-700 Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

17-700
12 assays  RIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      RIP
      Description
      Catalogue Number17-700
      Brand Family Upstate
      Trade Name
      • Magna RIP
      • Upstate
      DescriptionMagna RIP™ RNA-Binding Protein Immunoprecipitation Kit
      OverviewRNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq).

      Features & Benefits:
      -Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes
      -RNAse inhibitors and RNAse-free reagents
      -Negative controls
      Alternate Names
      • Magnetic RIP kit
      Materials Required but Not DeliveredMagna Grip™ Rack 8 well ( 20-400) (Now Available!) or similar magnetic rack.
      Background InformationGene regulation plays a critical role in complex cellular processes such as development, differentiation, and cellular response to environmental changes. In addition to transcriptional regulation of gene expression by transcription factors, cells utilize post-transcriptional regulatory mechanisms. One such mechanism involves use of certain RNA-binding proteins (RBPs) to temporally and coordinately regulate the rate of mRNA translation of functionally related gene
      products. While the regulation of gene expression by transcription factors has been well studied over time, the post-transcriptional regulation of mRNAs by RBPs and the role of non-coding RNAs in this process is a relatively nascent field that remains to be thoroughly explored.
      References
      Product Information
      Components
      • Magnetic Beads Protein A/G
      • RIP Wash Buffer
      • RIP Lysis Buffer
      • 0.5 M EDTA
      • 10% SDS
      • Salt Solution I
      • Salt Solution II
      • Precipitate Enhancer
      • Normal Mouse IgG
      • Rabbit IgG Purified
      • Protease Inhibitor Cocktail 200X
      • RNase Inhibitor
      • Proteinase K (10 mg/mL)
      • Nuclease free water
      PresentationTwo boxes containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions.
      Quality LevelMQ100
      Applications
      ApplicationRNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads.
      Key Applications
      • RNA Binding Protein Immunoprecipitation (RIP)
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.
      Packaging Information
      Material Size12 assays
      Material PackageRIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewApplicationPub Med ID
      LncRNA-PVT1 promotes pancreatic cancer cells proliferation and migration through acting as a molecular sponge to regulate miR-448.
      Zhao, L; Kong, H; Sun, H; Chen, Z; Chen, B; Zhou, M
      J Cell Physiol  233  4044-4055  2018

      Show Abstract
      28657147 28657147
      Linc00511 acts as a competing endogenous RNA to regulate VEGFA expression through sponging hsa-miR-29b-3p in pancreatic ductal adenocarcinoma.
      Zhao, X; Liu, Y; Li, Z; Zheng, S; Wang, Z; Li, W; Bi, Z; Li, L; Jiang, Y; Luo, Y; Lin, Q; Fu, Z; Rufu, C
      J Cell Mol Med  22  655-667  2018

      Show Abstract
      28984028 28984028
      LncRNA KCNQ1OT1 ameliorates particle-induced osteolysis through inducing macrophage polarization by inhibiting miR-21a-5p.
      Gao, X; Ge, J; Li, W; Zhou, W; Xu, L
      Biol Chem  399  375-386  2018

      Show Abstract
      29252185 29252185
      Neat1 regulates oxidized low-density lipoprotein-induced inflammation and lipid uptake in macrophages via paraspeckle formation.
      Huang-Fu, N; Cheng, JS; Wang, Y; Li, ZW; Wang, SH
      Mol Med Rep  17  3092-3098  2018

      Show Abstract
      29257236 29257236
      Auto- and cross-regulation of the hnRNPs D and DL.
      Kemmerer, K; Fischer, S; Weigand, JE
      RNA  24  324-331  2018

      Show Abstract
      29263134 29263134
      TUG1 knockdown ameliorates atherosclerosis via up-regulating the expression of miR-133a target gene FGF1.
      Zhang, L; Cheng, H; Yue, Y; Li, S; Zhang, D; He, R
      Cardiovasc Pathol  33  6-15  2018

      Show Abstract
      29268138 29268138
      AK098656, a Novel Vascular Smooth Muscle Cell-Dominant Long Noncoding RNA, Promotes Hypertension.
      Jin, L; Lin, X; Yang, L; Fan, X; Wang, W; Li, S; Li, J; Liu, X; Bao, M; Cui, X; Yang, J; Cui, Q; Geng, B; Cai, J
      Hypertension  71  262-272  2018

      Show Abstract
      29279317 29279317
      Long non-coding RNA DLEU1 predicts poor prognosis of gastric cancer and contributes to cell proliferation by epigenetically suppressing KLF2.
      Li, X; Li, Z; Liu, Z; Xiao, J; Yu, S; Song, Y
      Cancer Gene Ther  25  58-67  2018

      Show Abstract
      29282356 29282356
      LncRNA ZFAS1 promotes cell migration and invasion of fibroblast-like synoviocytes by suppression of miR-27a in rheumatoid arthritis.
      Ye, Y; Gao, X; Yang, N
      Hum Cell  31  14-21  2018

      Show Abstract
      28721682 28721682
      Effect of long non-coding RNA PVT1 on cell proliferation and migration in melanoma.
      Chen, L; Ma, D; Li, Y; Li, X; Zhao, L; Zhang, J; Song, Y
      Int J Mol Med  41  1275-1282  2018

      Show Abstract
      29286144 29286144

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)

      User Guides

      Title
      Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits