17-611 Magna ChIP™ G - Chromatin Immunoprecipitation Kit

17-611
22 assays  Kit capacity: 22 chromatin immunoprecipitation assays
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      Overview

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      For target-specific spike-in controls that make ChIP experiments more quantitative and accurate, Click on the Related Product & Applications tab above.
      Description
      Catalogue Number17-611
      Trade Name
      • Magna ChIP
      DescriptionMagna ChIP™ G - Chromatin Immunoprecipitation Kit
      OverviewChromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.

      Features & Benefits:
      Faster: Magnetic protein G beads allow for the entire ChIP protocol to be done in as little as a day! All reagents to process your samples are included - you don't have to spend valuable time making them.
      Easier: Spin columns make DNA purification easier and more reliable - no more messy phenol-chloroform extractions.
      Alternate Names
      • Magnetic ChIP Kit
      Materials Required but Not DeliveredMagna Grip™ Rack 8 well ( 20-400) (Now Available!) or similar magnetic rack.
      Background InformationChromatin Immunoprecipitation (ChIP) is a powerful technique for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits and post-translational modifications or other chromatin associated proteins such as transcription factors, chromatin regulators, etc. Additionally, ChIP can be used to identify regions of the genome associated with these proteins, or conversely, to identify proteins associated with a particular region of the genome. ChIP methodology often involves protein-DNA and protein-protein cross-linking, fragmentation of the cross-linked chromatin, and subsequent immunoprecipitation of chromatin with an antibody specific to a target protein. The DNA fragments isolated in complex with the target protein can be identified by a variety of methods including PCR, DNA microarray and DNA sequencing. Standard or quantitative PCR can be performed to verify whether a particular DNA sequence (the gene or region of the genome) is associated with the protein of interest. The combination of ChIP and promoter or genomic tiling microarrays (ChIP-chip) allows genome-wide identification of DNA-binding sites for chromatin-associated proteins with precise resolution. Alternatively, high-throughput sequencing of libraries constructed from immunoprecipitated chromosomal DNA (ChIP-Seq) is a powerful alternative to ChIP-chip in mapping the protein-DNA interactions across mammalian genomes.
      References
      Product Information
      Components
      • Magnetic Protein G Beads
      • ChIP Dilution Buffer
      • Low Salt Wash Buffer
      • High Salt Wash Buffer
      • LiCl Wash Buffer
      • TE Buffer
      • Cell Lysis Buffer
      • Nuclear Lysis Buffer
      • ChIP Elution Buffer (w/o Proteinase K)
      • 10X Glycine
      • 10X PBS
      • Protease Inhibitor Cocktail II
      • Proteinase K
      • Spin Filters
      • Collection Tubes
      • Bind Reagent A
      • Wash Reagent B
      • Elution Reagent C
      PresentationTwo boxes containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).
      Quality LevelMQ100
      Applications
      ApplicationSingle day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic G beads. Control primers included.
      Biological Information
      Analytes Available
      • Protein G
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
      Packaging Information
      Material Size22 assays
      Material PackageKit capacity: 22 chromatin immunoprecipitation assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Magna ChIP™ G - Chromatin Immunoprecipitation Kit SDS

      Title

      Safety Data Sheet (SDS) 

      Magna ChIP™ G - Chromatin Immunoprecipitation Kit Certificates of Analysis

      TitleLot Number
      Magna ChIP G Chromatin Immunoprecipitation Kits - DAM1439048DAM1439048
      Magna ChIP G or EZ-Magna ChIP G Chromatin Immunoprecipitation Kits -
      Magna ChIP G or EZ-Magna ChIP G Chromatin Immunoprecipitation Kits - 19631301963130
      Magna ChIP G or EZ-Magna ChIP G Chromatin Immunoprecipitation Kits - 19826761982676
      Magna ChIP G or EZ-Magna ChIP G Chromatin Immunoprecipitation Kits - 20296832029683
      Magna ChIP G or EZ-Magna ChIP G Chromatin Immunoprecipitation Kits - 20328592032859
      Magna ChIP G or EZ-Magna ChIP G Chromatin Immunoprecipitation Kits - 20551842055184
      Magna ChIP G or EZ-Magna ChIP G Chromatin Immunoprecipitation Kits - 20551862055186
      Magna ChIP G or EZ-Magna ChIP G Chromatin Immunoprecipitation Kits - 21919672191967
      Magna ChIP G or EZ-Magna ChIP G Chromatin Immunoprecipitation Kits - 22107642210764

      References

      Reference overviewPub Med ID
      Sleep loss reduces the DNA-binding of BMAL1, CLOCK, and NPAS2 to specific clock genes in the mouse cerebral cortex.
      Mongrain, Valérie, et al.
      PLoS ONE, 6: e26622 (2011)  2011

      Show Abstract
      22039518 22039518
      Molecular analysis of the JAZF1-JJAZ1 gene fusion by RT-PCR and fluorescence in situ hybridization in endometrial stromal neoplasms.
      Nucci, Marisa R, et al.
      Am. J. Surg. Pathol., 31: 65-70 (2007)  2007

      Show Abstract
      17197920 17197920
      Control of developmental regulators by Polycomb in human embryonic stem cells.
      Lee, Tong Ihn, et al.
      Cell, 125: 301-13 (2006)  2006

      Show Abstract
      16630818 16630818
      Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro.
      Kenji Furuno, Toshihiro Masatsugu, Miki Sonoda, Takehiko Sasazuki, Ken Yamamoto
      Biochemical and biophysical research communications  345  1051-8  2006

      Show Abstract
      16712789 16712789
      The use of chromatin immunoprecipitation assays in genome-wide analyses of histone modifications.
      Bernstein, Bradley E, et al.
      Meth. Enzymol., 376: 349-60 (2004)  2004

      14975317 14975317
      ChIP-chip: considerations for the design, analysis, and application of genome-wide chromatin immunoprecipitation experiments.
      Buck, Michael J and Lieb, Jason D
      Genomics, 83: 349-60 (2004)  2004

      Show Abstract
      14986705 14986705
      Molecular detection of JAZF1-JJAZ1 gene fusion in endometrial stromal neoplasms with classic and variant histology: evidence for genetic heterogeneity.
      Huang, Hsuan-Ying, et al.
      Am. J. Surg. Pathol., 28: 224-32 (2004)  2004

      Show Abstract
      15043312 15043312

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)

      FAQ

      QuestionAnswer
      How many PCR reactions can be done with this kit?There are enough primers and PCR buffer for 4 reactions per IP assuming a 20 microliter volume and assuming the primers are at the recommended concentration as stated in the manual.
      From where are the primer sequences derived for the kit?The primer sequences are based on the Human GAPDH promoter. The GenBank number is NT_009759.15, using nts:6497145-6498136.

      User Guides

      Title
      Magna ChIP¿ G

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      Categories

      Life Science Research > Kits & Assays > ChIP Kits > Kits and Assays
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Kits & Beads