GF053 Macrophage-Colony Stimulating Factor Protein, Recombinant human

GF053
10 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesSourcePurity
      CULT Human E. coli Greater than 98% by SDS-PAGE and HPLC analyses
      Description
      Catalogue NumberGF053
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionMacrophage-Colony Stimulating Factor Protein, Recombinant human
      OverviewM-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is involved in development, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells.

      Human M-CSF is a 36.8 kDa disulfide-linked homodimer composed of two 159 amino acid polypeptide subunits.
      Alternate Names
      • Macrophage colony-stimulating factor 1
      • CSF-1
      • M-CSF
      • MCSF
      • Lanimostim
      References
      Product Information
      PresentationThe sterile filtered solution was lyophilized from sodium phosphate, pH 8.0 and 75 mM NaCl.
      Quality LevelMQ100
      Applications
      ApplicationThe M-CSF protein is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts & osteoblasts.
      Key Applications
      • Cell Culture
      Application NotesFor most in vitro applications, M-CSF exerts its biological activity in the concentration range of 0.1 to 20.0 ng/mL.
      Biological Information
      PurityGreater than 98% by SDS-PAGE and HPLC analyses
      SourceE. coli
      Specific ActivityThe ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is < 5 ng/mL, corresponding to a specific activity of > 2 x 10<sup>5</sup> units/mg.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Four transcript variants encoding three different isoforms have been found for this gene.
      Gene Symbol
      • CSF1
      • M-CSF
      • MCSF
      • CSF-1
      • Lanimostim
      • MGC31930
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09603 # Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF- 1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
      SIZE: 554 amino acids; 60119 Da
      SUBUNIT: Homodimer or heterodimer; disulfide-linked.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein (By similarity). & Processed macrophage colony-stimulating factor 1: Secreted, extracellular space (By similarity).
      PTM: Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. & Isoform 1 is N- and O-glycosylated. Isoform 3 is N- glycosylated.
      Stem Cell Type
      • Hematopoietic Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized protein is stable for a few weeks at room temperature, but best stored at -20° C. After a quick spin, reconstitute in water to a concentration of 0.1 - 0.5 mg/mL. Reconstituted M-CSF should be stored in undiluted aliquots at -20° C. Avoid repeated freeze/thaw cycles. This solution can then be further diluted into other buffers and stored at 2° - 8° C for one week or stored at -20° C for future use.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Macrophage-Colony Stimulating Factor Protein, Recombinant human SDS

      Title

      Safety Data Sheet (SDS) 

      Macrophage-Colony Stimulating Factor Protein, Recombinant human Certificates of Analysis

      TitleLot Number
      RECOMBINANT HUMAN MACROPHAGE-COLONY STIMULATING FACTOR2919752
      RECOMBINANT HUMAN MACROPHAGE-COLONY STIMULATING FACTOR3135962
      RECOMBINANT HUMAN MACROPHAGE-COLONY STIMULATING FACTOR2984214
      RECOMBINANT HUMAN MACROPHAGE-COLONY STIMULATING FACTOR3046828
      RECOMBINANT HUMAN MACROPHAGE-COLONY STIMULATING FACTOR - 23904102390410
      RECOMBINANT HUMAN MACROPHAGE-COLONY STIMULATING FACTOR - 20253232025323
      RECOMBINANT HUMAN MACROPHAGE-COLONY STIMULATING FACTOR - 21726702172670
      RECOMBINANT HUMAN MACROPHAGE-COLONY STIMULATING FACTOR - 23147782314778
      RECOMBINANT HUMAN MACROPHAGE-COLONY STIMULATING FACTOR -24887802488780
      RECOMBINANT HUMAN MACROPHAGE-COLONY STIMULATING FACTOR -25028762502876

      Data Sheet

      Title
      RECOMBINANT HUMAN MACROPHAGE-COLONY STIMULATING FACTOR

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors