454850 MAP Kinase 2, Mouse, Recombinant, E. coli

454850
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454850-2000U
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      Description
      OverviewRecombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein kinase cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.
      Catalogue Number454850
      Brand Family Calbiochem®
      SynonymsERK2, MAPK2, p42MAPK
      References
      ReferencesFukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79.
      Wu, J., et al. 1993. Mol. Cell. Biol. 13, 4539.
      Product Information
      Activity≥5000 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na₂-EDTA, 50% glycerol, 0.01% BRIJ® 35 detergent, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Physicochemical Information
      ContaminantsMEK, phosphatase, protease: none detected.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MAP Kinase 2, Mouse, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      MAP Kinase 2, Mouse, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      454850

      References

      Reference overview
      Fukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79.
      Wu, J., et al. 1993. Mol. Cell. Biol. 13, 4539.

      Brochure

      Title
      MAPK Pathway Poster ( 750 KB )
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2008 RFH
      SynonymsERK2, MAPK2, p42MAPK
      DescriptionRecombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na₂-EDTA, 50% glycerol, 0.01% BRIJ® 35 detergent, pH 7.5.
      Recommended reaction conditions
      Note: This protocol is provided only as a general guide. Researchers should standardize this assay for their own specific needs and should consult published literature. Suggested Protocol to Assay for MAPK Activators 1. Combine the following on ice: 4.0 µl 250 mM HEPES, pH 7.5, 100 mM Mg(OAc)2, 500 µM ATP 2.0 µl MAP Kinase 1-29 µl purified MEK (cell lysates or fractions from chromatography) 2.0 µl [γ-32P]ATP (1.0 µCi/µl) add enough distilled H2O to produce a final volume of 40 µl. 2. Incubate at 30°C for 30 min. 3. Stop the reaction by adding 40 µl of 2X SDS-PAGE sample buffer. 4. The phosphorylated MAPK will show as a signal at ~45 kDa by autoradiography. Phosphorylating Protein Substrates with Phosphorylated MAPK 1. Combine the following on ice: 5.0 µl 500 mM Tris-HCl (pH 7.5), 62.5 mM β-glycerol phosphate, 50 mM MgCl2, 5 mM EGTA, 250 µM NaF, 5 mM DTT, and 2.5 mM sodium vanadate 2 µg experimental protein or Myelin Basic Protein Peptide Substrate (Cat. No. 475920) 1.0 µl phosphorylated MAPK 2.0 µl [γ-32P]ATP (1.25 mM,100 µCi/µmoll) add enough distilled H2O to produce a final volume of 25 µl. 2. Incubate at 30°C for 30 min. 3. Stop the reaction by spotting 20 µl of each reaction mixture onto a 1.5 cm2 piece of Whatman P81 paper. 4. Wash the squares 7X for 5 min with phosphoric acid (1% w/v). 5. Quantitate the 32P incorporation in a liquid scintillation counter.
      Purity≥90% by SDS-PAGE
      ContaminantsMEK, phosphatase, protease: none detected.
      Activity≥5000 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesFukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79.
      Wu, J., et al. 1993. Mol. Cell. Biol. 13, 4539.